FastQCFastQC Report
Tue 16 Jan 2018
H5TH7BCX2_l02n01_gal-ssb_gal_dox-3.351000000b0e9a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TH7BCX2_l02n01_gal-ssb_gal_dox-3.351000000b0e9a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28204596
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC357280012.66743902305851TruSeq Adapter, Index 9 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACAC2002200.7098843039623756TruSeq Adapter, Index 14 (97% over 38bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGA952260.3376258252378442TruSeq Adapter, Index 9 (100% over 40bp)
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCA912650.3235820147893627TruSeq Adapter, Index 9 (100% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGAACTCGTATGC710470.25189866218966583TruSeq Adapter, Index 9 (98% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGACCTCGTATGC657350.2330648522673397TruSeq Adapter, Index 9 (98% over 50bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG582070.20637416682018775TruSeq Adapter, Index 9 (100% over 49bp)
CTCTTCCGATCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCA535970.1900293129531088TruSeq Adapter, Index 9 (100% over 38bp)
AGGTCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC362530.12853578898985116TruSeq Adapter, Index 9 (98% over 50bp)
ACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAG338730.12009744794784509TruSeq Adapter, Index 9 (100% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC316650.11226893659458906TruSeq Adapter, Index 9 (100% over 51bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCAGATCGGAAGAGCACACG313660.11120882568216896Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC4316550.042.13990445
TCGTATG4442600.040.89668344
CTCGTAT4437200.040.6552443
TCTCGTA4280000.040.21946342
ATCTCGT4288650.040.11107641
GATCTCG4341200.039.4421440
AGATCTC5128750.033.23515339
CAGATCT5148200.032.929438
AGGTCGG64700.031.878021
GGTCGGA64900.031.7849332
GACCTCG103350.031.53185540
CCTCGTA104700.031.38304942
ACCTCGT105600.031.28596141
ACTCGTA120700.030.70729342
ATCAGAT5554050.030.53897336
TCAGATC5599600.030.2667837
GAACTCG123950.029.70255340
AACTCGT128400.028.55053141
GTCACGC29250.027.98456630
GACGTGT611900.027.6564271
Produced by FastQC (version 0.11.2)