FastQCFastQC Report
Tue 16 Jan 2018
H5TH7BCX2_l02n01_gal-ssb_gal_dox-1.351000000b0e57.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TH7BCX2_l02n01_gal-ssb_gal_dox-1.351000000b0e57.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11793622
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC8907807.553065546784525TruSeq Adapter, Index 7 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACAC682890.5790333113949218TruSeq Adapter, Index 14 (97% over 38bp)
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGAT243450.20642513385624875TruSeq Adapter, Index 7 (100% over 38bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCA220170.18668565093912626TruSeq Adapter, Index 7 (100% over 40bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG138700.1176059398885262TruSeq Adapter, Index 7 (100% over 49bp)
CTCTTCCGATCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGAT136080.11538440014441703TruSeq Adapter, Index 7 (100% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCACCTCGTATGC132430.11228950698945583TruSeq Adapter, Index 7 (98% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCAACTCGTATGC124220.10532811718062525TruSeq Adapter, Index 7 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1084200.041.59584845
TCGTATG1120650.040.2273344
CTCGTAT1122700.039.94959343
CATCTCG1082650.039.5163240
ATCTCGT1098200.039.30697641
TCTCGTA1100350.039.2526642
TCATCTC1278800.033.1525839
GATCATC1284250.032.70635237
ATCATCT1306900.032.28777738
AGATCAT1343800.031.3277536
CAGATCA1568950.027.83245335
CACCTCG27500.026.16994940
GGTCGGA19650.025.7593422
AGGTCGG19350.025.714711
GACGTGT152350.024.7921351
CCTCGTA29400.024.78467642
ACCTCGT29500.024.77689741
TCACCAG1835450.024.16161231
ACCAGAT1820750.024.15824933
CACCAGA1837850.024.07744632
Produced by FastQC (version 0.11.2)