FastQCFastQC Report
Tue 16 Jan 2018
H5TH7BCX2_l02n01_exo_hku-2.351000000b0d8e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TH7BCX2_l02n01_exo_hku-2.351000000b0d8e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12555949
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTATGC7814676.223878418110809TruSeq Adapter, Index 22 (98% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACAC1987941.5832654305938962TruSeq Adapter, Index 14 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTATGCC526510.41933110750927705TruSeq Adapter, Index 22 (98% over 51bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGAACTCGTATGC194290.15473939882998888TruSeq Adapter, Index 22 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCAGATCGGAAGAGCACACG170080.13545770216174022Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGACCTCGTATGC151200.12042100521434103TruSeq Adapter, Index 22 (97% over 39bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTATG138000.10990806031467633TruSeq Adapter, Index 22 (97% over 49bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1029750.039.01247445
TCGTATG1071950.037.3876544
CTCGTAT1068500.037.13596343
ATCTCGT1012900.037.0407541
TCTCGTA1020650.036.7859342
GATCTCG1040300.035.82518840
ACGATCT1069950.034.40308838
TACGATC1075100.034.22573537
GTACGAT1094150.033.6981136
CGTACGA1190550.032.73561535
CCGTACG1225550.031.86665734
ACCGTAC1269850.030.8610933
CACCGTA1286000.030.56921432
CGATCTC1214350.030.47562439
GTCACCG1303600.030.25999830
TCACCGT1307700.030.17372331
AGTCACC1338250.030.00749229
CAGTCAC1714400.029.77472928
CCAGTCA1741150.029.52521927
TCCAGTC1811750.029.12568326
Produced by FastQC (version 0.11.2)