FastQCFastQC Report
Tue 16 Jan 2018
H5TH7BCX2_l02n01_exo_hku-1.351000000b0d71.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TH7BCX2_l02n01_exo_hku-1.351000000b0d71.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11766765
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTCGTATGC5904515.017955232385452TruSeq Adapter, Index 20 (98% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACAC787410.6691813765295729TruSeq Adapter, Index 14 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTCGTATGCC308230.2619496522621128TruSeq Adapter, Index 20 (98% over 51bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCAGATCGGAAGAGCACACG176420.14993075836901645Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC765050.038.8069945
TCGTATG789150.037.5520344
CTCGTAT786900.037.43602843
CATCTCG746150.037.31648640
ATCTCGT760250.037.07106441
TCTCGTA777100.036.3338142
GCCATCT824650.033.38189338
GGCCATC817450.033.3210337
CCATCTC858200.032.3106539
TGGCCAT848100.032.24231736
GTGGCCA905950.031.47407235
CGTGGCC938950.030.42310734
GTGACTG47350.030.3324171
ACGTGGC971400.029.62403933
CACGTGG982350.029.3645432
TCACGTG997900.029.01287331
GTCACGT1015600.028.96777730
AGTCACG1028150.028.84626629
CAGTCAC1224600.028.28428628
CCAGTCA1250850.027.98412527
Produced by FastQC (version 0.11.2)