FastQCFastQC Report
Tue 16 Jan 2018
H5TH7BCX2_l02n01_exo1d-3.351000000b0e4a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TH7BCX2_l02n01_exo1d-3.351000000b0e4a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9244664
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC2535332.742479337269586TruSeq Adapter, Index 6 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACAC113250.12250310016675567TruSeq Adapter, Index 14 (97% over 38bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC324050.040.02211445
TATCTCG317400.038.8430240
TCGTATG335800.038.64011844
CTCGTAT334850.038.5650443
ATCTCGT326950.038.27936641
TCTCGTA337050.037.23904842
ATATCTC388250.031.78369539
CAATATC409650.029.76018137
AATATCT413300.029.68252638
CCAATAT422650.028.79990436
GCCAATA471600.026.57352435
CGCCAAT495450.025.41673734
ACGCCAA520550.024.35075633
CACGCCA523200.024.21869332
TCACGCC528550.024.11394731
GTCACGC535250.024.05576530
AGTCACG547000.023.70783229
CAGTCAC596300.022.82627928
CCAGTCA613450.022.2750227
TCCAGTC624450.022.138326
Produced by FastQC (version 0.11.2)