FastQCFastQC Report
Tue 16 Jan 2018
H5TH7BCX2_l02n01_exo1d-2.351000000b0e20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TH7BCX2_l02n01_exo1d-2.351000000b0e20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11733291
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC2249301.9170239619898628TruSeq Adapter, Index 5 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACAC353080.30092154025669354TruSeq Adapter, Index 14 (97% over 38bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC292600.039.71154845
TCGTATG303650.038.22890544
CTCGTAT302900.037.94009443
GATCTCG292000.037.62343640
ATCTCGT294800.037.44155541
TCTCGTA305950.036.15789442
GTGATCT358600.030.12691538
AGTGATC358700.029.96177737
TGATCTC367000.029.57318139
CAGTGAT386650.027.95710236
ACAGTGA436150.025.67096935
CACAGTG454500.024.65421534
ACACAGT471800.023.79298833
CACACAG477100.023.67448632
GTCACAC484650.023.65830230
AGTCACA569900.023.52073929
CAGTCAC577600.023.47970428
CCAGTCA588700.023.1005527
TCCAGTC599000.023.08256526
TCACACA493300.022.97450831
Produced by FastQC (version 0.11.2)