FastQCFastQC Report
Tue 16 Jan 2018
H5TH7BCX2_l02n01_dna2-frb-3.351000000b0dde.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TH7BCX2_l02n01_dna2-frb-3.351000000b0dde.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13097956
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC2213351.6898438199059456TruSeq Adapter, Index 3 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACAC235640.17990593341434344TruSeq Adapter, Index 14 (97% over 38bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC297550.037.9636245
TCGTATG308450.036.55547344
CTCGTAT309600.036.3633843
CATCTCG295950.036.33078440
ATCTCGT305850.035.47833341
TCTCGTA319850.034.0660542
GCATCTC385900.027.76914639
GGCATCT387650.027.38230138
AGGCATC387200.027.31528337
TAGGCAT401200.026.4057436
TTAGGCA448250.024.37137635
CTTAGGC467000.023.38787534
GACGTGT71200.022.7123131
ACTTAGG493000.022.25453233
CACTTAG524450.021.0355132
CAGTCAC606400.020.92209428
TCACTTA533550.020.84110631
AGTCACT542650.020.77761829
GTCACTT539850.020.68953130
TCCAGTC633150.020.41407826
Produced by FastQC (version 0.11.2)