FastQCFastQC Report
Tue 16 Jan 2018
H5TH7BCX2_l02n01_dna2-frb-2.351000000b0dc1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TH7BCX2_l02n01_dna2-frb-2.351000000b0dc1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9682447
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC1441111.4883737551055016TruSeq Adapter, Index 2 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACAC262180.27077865750259206TruSeq Adapter, Index 14 (97% over 38bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC198450.036.91961745
CTCGTAT205350.035.50458543
TCGTATG206200.035.48708744
TATCTCG197600.035.0078640
ATCTCGT203650.034.4647841
TCTCGTA214950.032.694842
ATGTATC255700.026.64868537
GTATCTC264100.026.2440239
TGTATCT271250.025.40287638
GATGTAT270550.025.1932636
CGATGTA297200.023.79663735
CCGATGT307850.022.99519334
ACCGATG329250.021.6371933
GACGTGT50300.021.4065721
CAGTCAC412300.021.16193428
CACCGAT339300.021.03584932
TCACCGA341700.020.99997531
GTCACCG345200.020.943430
AGTCACC354300.020.71013329
TCCAGTC432700.020.55793626
Produced by FastQC (version 0.11.2)