FastQCFastQC Report
Tue 16 Jan 2018
H5TH7BCX2_l01n02_wt-hu-3.352000000b0e3a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TH7BCX2_l01n02_wt-hu-3.352000000b0e3a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17164432
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG2307611.3444138436972457Illumina Single End PCR Primer 1 (100% over 51bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT223530.1302286029622186Illumina Single End PCR Primer 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG274850.039.5330518
CACACGT203150.038.88053519
ACACGTC142600.038.33324420
AGCACAC300050.037.73663317
GTCGCCG348000.037.59502845
GAGCACA313100.037.09647816
AGAGCAC316400.036.76597615
GGTCGCC357600.036.4723344
CACGTCT77300.035.66326521
GTGGTCG370500.034.7766742
ACGTCAG75300.034.49031422
ACACGAG85250.034.47397220
CACGAGA86250.033.99606321
TGGTCGC384400.033.54231643
CACACGA90100.033.06450319
CACGTAG69650.033.05894521
GGTGGTC392800.032.806341
CTCGGTG390800.032.68628738
CGTCTGA61750.032.44523223
CGGTGGT401350.032.0512640
Produced by FastQC (version 0.11.2)