FastQCFastQC Report
Tue 16 Jan 2018
H5TH7BCX2_l01n02_wt-hu-2.352000000b0e10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TH7BCX2_l01n02_wt-hu-2.352000000b0e10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2687568
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1016153.7809275895530825Illumina Single End PCR Primer 1 (100% over 51bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT104490.3887901627047204Illumina Single End PCR Primer 1 (100% over 51bp)
AGATGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT29230.10876003881576206Illumina Single End PCR Primer 1 (100% over 47bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC60400.040.47268320
CACACGT88150.040.33095619
GCACACG122150.040.1674418
AGCACAC129500.039.060417
ACGTCAG31450.038.97133322
GAGCACA133850.038.83160416
CACGTCT30950.038.72897321
GTCGCCG161550.038.69201345
AGAGCAC135250.038.51132215
CGTCTGA23600.038.30747223
CACGTAG28850.038.2741121
TCGCCGT22200.038.1917845
ACGTCTG24400.037.97316722
ACACGTA29600.037.53225720
CACGTCA33100.037.50425321
ACACGAG35300.037.46036520
CACGAGA35400.037.03690721
GGTCGCC168150.036.90580444
CGTCAGA33200.036.71390523
CACACGA36700.036.5181919
Produced by FastQC (version 0.11.2)