FastQCFastQC Report
Tue 16 Jan 2018
H5TH7BCX2_l01n02_wt-3.352000000b0de8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TH7BCX2_l01n02_wt-3.352000000b0de8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7548181
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG2990913.962424854411944Illumina Single End PCR Primer 1 (100% over 51bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT199110.26378540737165684Illumina Single End PCR Primer 1 (100% over 51bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG297100.040.45337718
CACACGT205700.040.2351919
ACACGTC137450.040.18213320
AGCACAC314800.039.97374717
GAGCACA326950.039.6152916
GTCGCCG423350.039.4841645
AGAGCAC329200.039.45315
GGTCGCC435600.038.36342644
ACGTCAG79400.038.12184522
CACGTAG71150.038.08566321
ACACGAG97500.038.0795520
TGGTCGC438400.037.8199643
CACACGA99100.037.66248719
GTGGTCG441550.037.5705942
CACGTCT66050.037.41745421
CACGAGA99650.037.121
CGTCTGA47050.036.5159923
GGTGGTC456800.036.3404441
ACACGTA75550.036.31405620
AAGAGCA363650.036.197614
Produced by FastQC (version 0.11.2)