FastQCFastQC Report
Tue 16 Jan 2018
H5TH7BCX2_l01n02_wt-2.352000000b0db1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TH7BCX2_l01n02_wt-2.352000000b0db1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8598248
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG2603213.027605158632317Illumina Single End PCR Primer 1 (100% over 51bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT182080.21176407100609332Illumina Single End PCR Primer 1 (100% over 51bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG264900.040.57904418
CACACGT187450.039.8734219
AGCACAC280300.039.676517
GAGCACA289500.039.36985416
ACACGTC126400.039.31979820
AGAGCAC291050.039.2905815
GTCGCCG381050.038.65684545
ACACGAG85100.037.97460620
ACGTCAG74550.037.76789522
GGTCGCC394500.037.41914444
CACGAGA86400.037.1429421
CACACGA88400.036.7810419
CACGTAG65400.036.69042621
TGGTCGC399450.036.61101543
GTGGTCG402150.036.3432142
CACGTCT59000.035.6390121
ACACGTA68650.035.60861620
AAGAGCA326000.035.42307714
CACGTCA80100.035.29139321
GGTGGTC416300.035.12173541
Produced by FastQC (version 0.11.2)