FastQCFastQC Report
Tue 16 Jan 2018
H5TH7BCX2_l01n02_wt-1.352000000b0d98.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TH7BCX2_l01n02_wt-1.352000000b0d98.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11414441
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG3861643.3831179292967564Illumina Single End PCR Primer 1 (100% over 51bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT269030.23569266335513056Illumina Single End PCR Primer 1 (100% over 51bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG362250.040.17989718
CACACGT256350.039.91858319
AGCACAC384300.039.6030417
ACACGTC171450.039.49234420
GTCGCCG587850.039.46216245
AGAGCAC401450.038.9571815
GAGCACA400650.038.95675316
GGTCGCC603000.038.52307544
TGGTCGC608350.037.71775443
GTGGTCG610350.037.54258342
ACGTCAG104450.037.37497322
CACGTAG90750.037.36752721
CACACGA117450.036.7003919
ACACGAG116550.036.67832620
GGTGGTC629750.036.45018441
CACGTCT76850.036.28419521
ACACGTA94800.036.26926820
CACGAGA118950.035.86266321
CACGTCA109150.035.70380421
CGTCAGA111650.035.0654723
Produced by FastQC (version 0.11.2)