FastQCFastQC Report
Tue 16 Jan 2018
H5TH7BCX2_l01n02_gal-ssb_glu_doxrap-4.352000000b0f1f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TH7BCX2_l01n02_gal-ssb_glu_doxrap-4.352000000b0f1f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26078
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1630.6250479331237059Illumina Single End PCR Primer 1 (100% over 51bp)
GTGACTGGAGTTCAGACGTGTGCTCTTCCGATAGATCGGAAGAGCGTCGTG400.15338599585857812Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
GTGACTGGAGTTCAGACGTGTGCTAGATCGGAAGAGCGTCGTGTAGGGAAA320.12270879668686248Illumina Single End PCR Primer 1 (100% over 27bp)
GTGACTGGAGTTCAGACGTGTGCTCTTCAGATCGGAAGAGCGTCGTGTAGG290.11120484699746913Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTCTG253.7903E-544.992321
CACGTCT253.7903E-544.9923221
GGTCTGA206.912757E-444.992322
CATCCGG206.912757E-444.9923213
TCTACCG206.912757E-444.9923224
GAGCATC302.087112E-644.9923210
AGAGCAT351.1535303E-744.992329
CGGGAGA206.912757E-444.992325
TCTGAAC206.912757E-444.9923225
GGGACTA206.912757E-444.992321
AGCATCC206.912757E-444.9923211
ATCCGGT206.912757E-444.9923214
GCATCCG206.912757E-444.9923212
GTGTGCA206.912757E-444.9923218
CGACTGG206.912757E-444.992322
GTCGGGA206.912757E-444.992323
TCGGGAG206.912757E-444.992324
TGACTGG2000.043.867512
GTGACTG2000.043.867511
CTGGAGT2350.043.0777555
Produced by FastQC (version 0.11.2)