FastQCFastQC Report
Tue 16 Jan 2018
H5TH7BCX2_l01n02_gal-ssb_glu_doxrap-3.352000000b0ef4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TH7BCX2_l01n02_gal-ssb_glu_doxrap-3.352000000b0ef4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5762
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG80.13884068031933355Illumina Single End PCR Primer 1 (100% over 51bp)
AAGTGACTGGAGTTCAGACGTGTGCAGATCGGAAGAGCGTCGTGTAGGGAA60.10413051023950018Illumina Single End PCR Primer 1 (100% over 26bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT206.463368E-445.019
AAGAGCA206.463368E-445.014
AGAGCAC206.463368E-445.015
AGCACAC206.463368E-445.017
GCACACG206.463368E-445.018
GAGCACA250.001937885236.016
Produced by FastQC (version 0.11.2)