FastQCFastQC Report
Tue 16 Jan 2018
H5TH7BCX2_l01n02_gal-ssb_glu_doxrap-2.352000000b0ee7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TH7BCX2_l01n02_gal-ssb_glu_doxrap-2.352000000b0ee7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9730794
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG6123796.293206905829061Illumina Single End PCR Primer 1 (100% over 51bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT322700.3316276143550054Illumina Single End PCR Primer 1 (100% over 51bp)
GAGGAGAACTTCTAGTATATTCTGTATACCTAATATTATAGCCTTTATCAA147810.15189921809052787No Hit
AGATGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT133910.13761466947095993Illumina Single End PCR Primer 1 (100% over 47bp)
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC126460.12995856247701884Illumina Single End PCR Primer 1 (100% over 50bp)
AGGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC113120.11624950646370687Illumina Single End PCR Primer 1 (98% over 50bp)
AGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG112910.11603369673636088Illumina Single End PCR Primer 1 (98% over 51bp)
AGACGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT98200.10091673916845839Illumina Single End PCR Primer 1 (97% over 49bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG463000.042.20763418
GAGCACA646300.041.82674816
AGCACAC500000.041.77342217
ACACGAG254700.041.68914420
CACACGA255100.041.61132419
AGAGCAC654550.041.505215
CACGAGA258750.040.99316421
CACACGT218100.040.5467719
GTCGCCG884950.040.37320345
CACGTAG111650.040.03903221
ACACGTA115500.039.30793420
ACACGTC108150.038.7773620
ACGAGAT274900.038.6421322
TCGCCGT87850.038.5874945
AAGAGCA816850.038.34156414
GGTCGCC936450.038.11434644
GCACAAG138650.037.7498618
GTGGTCG942900.037.17926442
TGGTCGC946200.037.07085843
CACGTCT56650.036.71704521
Produced by FastQC (version 0.11.2)