FastQCFastQC Report
Tue 16 Jan 2018
H5TH7BCX2_l01n02_gal-ssb_gal_doxrap-2.352000000b0e8a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TH7BCX2_l01n02_gal-ssb_gal_doxrap-2.352000000b0e8a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11731792
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG226910019.3414612192238Illumina Single End PCR Primer 1 (100% over 51bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT1786511.5227937897296508Illumina Single End PCR Primer 1 (100% over 51bp)
AGATGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT688050.5864832925779795Illumina Single End PCR Primer 1 (100% over 47bp)
AGACCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG489820.4175150735710282Illumina Single End PCR Primer 1 (98% over 51bp)
AGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG415650.3542937003997343Illumina Single End PCR Primer 1 (98% over 51bp)
AGGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC398210.339428111238249Illumina Single End PCR Primer 1 (98% over 50bp)
AGACGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT348440.29700492473784057Illumina Single End PCR Primer 1 (97% over 49bp)
GGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG344530.29367210056230114Illumina Single End PCR Primer 1 (98% over 51bp)
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC301050.25661041382254307Illumina Single End PCR Primer 1 (100% over 50bp)
AGAGCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG286840.24449802724085118Illumina Single End PCR Primer 1 (98% over 51bp)
AGGGCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG264410.2253790384282299Illumina Single End PCR Primer 1 (100% over 47bp)
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC228370.1946590938536926Illumina Single End PCR Primer 1 (100% over 50bp)
TAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC192630.16419486468904324Illumina Single End PCR Primer 1 (100% over 50bp)
GGCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCGGAAGAGCGTCGTGT181880.1550317291680589Illumina Single End PCR Primer 1 (97% over 34bp)
AGATAGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG157690.13441254328409505Illumina Single End PCR Primer 1 (98% over 51bp)
AGATTGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG153100.13050009751280964Illumina Single End PCR Primer 1 (98% over 51bp)
GAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCAT152650.13011652439797772Illumina Single End PCR Primer 1 (100% over 51bp)
AGTTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG151870.1294516643322691Illumina Single End PCR Primer 1 (98% over 51bp)
AGAGTGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG136750.11656360767391717Illumina Single End PCR Primer 1 (100% over 46bp)
GGGCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCGGAAGAGCGTCGTG135970.11589874760820854Illumina Single End PCR Primer 1 (97% over 34bp)
AGAACGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG129450.11034119936664406Illumina Single End PCR Primer 1 (98% over 51bp)
AAATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG125680.10712770904905235Illumina Single End PCR Primer 1 (98% over 51bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC602950.041.45853820
CGTCTGA229900.041.4444423
CACGTCT290750.041.36360521
ACGTCTG233700.041.29977822
CACACGT932850.041.2813419
GCACACG1360500.041.04535718
AGCACAC1410900.040.81726517
GAGCACA1446700.040.7324516
AGAGCAC1454300.040.6420315
ACGTCAG315500.040.5991922
CACGTAG332850.040.320621
TCGCCGT387300.040.2524445
ACACGTA337050.040.16510420
CACGTCA319550.040.1409321
ACACGAG431600.039.7027220
CACACGA434000.039.60414519
GTCGCCG3231150.039.5055745
AAGAGCA1512500.039.33080714
CACGAGA436300.039.2904921
CGTCAGA327950.039.06477723
Produced by FastQC (version 0.11.2)