FastQCFastQC Report
Tue 16 Jan 2018
H5TH7BCX2_l01n02_gal-ssb_gal_doxrap-1.352000000b0e60.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TH7BCX2_l01n02_gal-ssb_gal_doxrap-1.352000000b0e60.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12049509
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG178063214.77763118812559Illumina Single End PCR Primer 1 (100% over 51bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT1336941.1095389861943752Illumina Single End PCR Primer 1 (100% over 51bp)
AGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG341380.2833144487464178Illumina Single End PCR Primer 1 (98% over 51bp)
AGATGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT306490.25435891205193506Illumina Single End PCR Primer 1 (100% over 47bp)
GGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG280940.23315472854537062Illumina Single End PCR Primer 1 (98% over 51bp)
AGAGCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG260610.21628267176695748Illumina Single End PCR Primer 1 (98% over 51bp)
AGACCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG236060.19590839759528791Illumina Single End PCR Primer 1 (98% over 51bp)
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC201640.16734291828820577Illumina Single End PCR Primer 1 (100% over 50bp)
AGGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC197740.16410627188211568Illumina Single End PCR Primer 1 (98% over 50bp)
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC156360.12976462360416513Illumina Single End PCR Primer 1 (100% over 50bp)
AGGGCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG149210.12383077185966665Illumina Single End PCR Primer 1 (100% over 47bp)
AGACGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT144500.11992189889231172Illumina Single End PCR Primer 1 (97% over 49bp)
TAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC134450.11158131007661806Illumina Single End PCR Primer 1 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC703200.041.28721220
CACGTCT290150.040.83571621
CACACGT1051000.040.77039719
CGTCTGA212450.040.58167323
ACGTCAG418850.040.56654422
GCACACG1566950.040.3350718
GTCGCCG2552100.040.30288745
ACGTCTG217850.040.2983122
AGCACAC1644400.040.19229517
TCGCCGT257250.040.1761845
CACGTCA422600.040.1427221
GAGCACA1702750.039.98586316
AGAGCAC1707800.039.9429715
CGTCAGA431650.039.4469523
CACGTAG349300.039.1159421
ACACGAG515350.038.89620
ACACGTA355450.038.88220
CACACGA517900.038.8701119
GGTCGCC2648200.038.85141444
AAGAGCA1789350.038.56602514
Produced by FastQC (version 0.11.2)