FastQCFastQC Report
Tue 16 Jan 2018
H5TH7BCX2_l01n01_wt-hu-3.351000000b0e3d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TH7BCX2_l01n01_wt-hu-3.351000000b0e3d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17164432
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC2554951.4885141553183934TruSeq Adapter, Index 10 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCC238200.13877534660045843TruSeq Adapter, Index 10 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC349250.034.89888445
TATCTCG353650.033.4402440
CTCGTAT364850.033.3321443
TCGTATG365800.033.31926344
ATCTCGT368850.032.38528441
TCTCGTA388000.030.86813242
GCTTATC389100.030.13354337
TAGCTTA399450.029.81924235
CACTAGC412900.029.20221332
AGCTTAT402450.029.10516736
ACTAGCT414850.028.86977633
TTATCTC412400.028.56730539
CTAGCTT418400.028.49580834
TCACTAG425050.028.42559631
AGTCACT429450.028.3176629
CAGTCAC460300.028.06642228
CCAGTCA468500.027.82441327
TCCAGTC477650.027.69099426
CTTATCT427850.027.55156938
GTCACTA447700.027.07791930
Produced by FastQC (version 0.11.2)