FastQCFastQC Report
Tue 16 Jan 2018
H5TH7BCX2_l01n01_wt-hu-2.351000000b0e13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TH7BCX2_l01n01_wt-hu-2.351000000b0e13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2687568
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC1150674.2814544599429665TruSeq Adapter, Index 9 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC110140.4098128865948694TruSeq Adapter, Index 9 (100% over 51bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACAC59690.22209670601822915TruSeq Adapter, Index 14 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC161900.035.66204545
TCGTATG169150.034.11958744
CTCGTAT169150.033.9733243
GTGACTG22700.033.3351
ATCTCGT166950.033.1956741
TCTCGTA168700.032.8646542
GATCTCG175900.031.37935340
AGATCTC194250.028.32244139
CAGATCT198850.027.62252638
TGACTGG27300.027.5228042
CGATCAG207700.027.3547134
GACTGGA28000.027.2376583
GATCAGA209500.027.1196835
GTCACGA213800.027.09975230
CACGATC212400.027.0776832
AGTCACG215550.026.99451829
TCACGAT214550.026.88970431
ACGATCA214100.026.67357833
CAGTCAC234600.026.52823328
ATCAGAT207700.026.46671136
Produced by FastQC (version 0.11.2)