FastQCFastQC Report
Tue 16 Jan 2018
H5TH7BCX2_l01n01_wt-3.351000000b0deb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TH7BCX2_l01n01_wt-3.351000000b0deb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7548181
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATGC3155004.179814977939718TruSeq Adapter, Index 16 (98% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACAC223030.29547516149917447TruSeq Adapter, Index 14 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATGCC213570.28294234067784013TruSeq Adapter, Index 16 (98% over 51bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC418500.036.8211445
TCGTATG433500.035.55153744
CTCGTAT433450.035.49360743
ATCTCGT429850.034.95888541
TCTCGTA431550.034.8787342
CATCTCG429650.034.75528740
GTCCATC486250.030.49715837
CGTCCAT491450.030.16451536
CCGTCCA507000.029.9136635
CCATCTC500350.029.72295239
GTGACTG31800.029.48221
TCCATCT504900.029.45529638
CCCGTCC529050.028.67134734
ACCCGTC533950.028.57251533
CACCCGT539050.028.36894832
TCACCCG541550.028.23364631
GTCACCC550000.027.99616830
AGTCACC560150.027.81009929
CAGTCAC615950.027.33560828
CCAGTCA623350.027.08272427
Produced by FastQC (version 0.11.2)