FastQCFastQC Report
Tue 16 Jan 2018
H5TH7BCX2_l01n01_wt-2.351000000b0db4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TH7BCX2_l01n01_wt-2.351000000b0db4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8598248
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTCGTATGC2766113.217062359680716TruSeq Adapter, Index 15 (98% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACAC232340.2702178397273491TruSeq Adapter, Index 14 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTCGTATGCC194610.2263368072193312TruSeq Adapter, Index 15 (98% over 51bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC397600.034.27090545
TCGTATG414900.032.85161244
CTCGTAT414150.032.77552443
TCTCGTA413550.032.01288642
ATCTCGT414050.031.92496541
AATCTCG420650.031.0446140
GTGACTG28050.026.1763671
CTACACG34900.025.97059612
GTCAATC512350.025.35671837
CAATCTC514400.025.29924239
CGACGCT35400.025.2225817
TCAATCT530800.024.58975838
TGTCAAT535950.024.38199836
ACGACGC37150.024.27659816
CCTACAC37750.024.18863511
CCCTACA37850.024.18414910
ACACGAC37850.024.1247314
ATGTCAA558050.023.98872835
TCACATG563100.023.95333131
ACATGTC561150.023.94853233
Produced by FastQC (version 0.11.2)