FastQCFastQC Report
Tue 16 Jan 2018
H5TH7BCX2_l01n01_wt-1.351000000b0d9b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TH7BCX2_l01n01_wt-1.351000000b0d9b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11414441
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTATGC4115593.605599257992573TruSeq Adapter, Index 14 (98% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACAC741860.6499310829150547TruSeq Adapter, Index 14 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTATGCC292840.2565522043523638TruSeq Adapter, Index 14 (98% over 51bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC543600.036.875545
TCGTATG561500.035.6876244
CTCGTAT559800.035.63125643
TCTCGTA558850.034.90699442
ATCTCGT562100.034.68100741
CATCTCG560800.034.52536840
CCATCTC674800.028.55602539
GTTCCAT677150.028.42283636
TCCATCT687250.028.05847438
AGTTCCA702150.028.02908535
TTCCATC696450.027.60385737
GTCACAG870750.027.04445630
ACAGTTC732200.026.97988533
CAGTTCC729600.026.97453534
TCACAGT736700.026.92487731
AGTCACA878000.026.89286629
CACAGTT740500.026.792932
CAGTCAC890800.026.78165428
CCAGTCA899650.026.60785327
TCCAGTC909850.026.51921726
Produced by FastQC (version 0.11.2)