FastQCFastQC Report
Tue 16 Jan 2018
H5TH7BCX2_l01n01_gal-ssb_glu_doxrap-4.351000000b0f12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TH7BCX2_l01n01_gal-ssb_glu_doxrap-4.351000000b0f12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26078
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACAC10383.980366592530102TruSeq Adapter, Index 14 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACG1660.6365518828130992TruSeq Adapter, Index 14 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCAATCACGATCTCGTATGCC1240.47549658716159215TruSeq Adapter, Index 3 (97% over 34bp)
GAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGA480.18406319503029372TruSeq Adapter, Index 14 (96% over 33bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGT410.15722064575504258TruSeq Adapter, Index 14 (97% over 37bp)
AGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTC350.13421274637625585TruSeq Adapter, Index 14 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACTGG401.4508058E-533.9237442
GTGACTG401.4508058E-533.9237441
GACTGGA453.2464457E-530.1544423
TGGAGTT453.3777644E-529.9808066
ACTGGAG506.659537E-527.1389964
ACAGATC1900.024.85251233
CTGGAGT551.2732937E-424.6718145
GGAGTTC551.3245034E-424.5297497
CACAGAT1950.024.21526532
AGTCACA1900.023.6690629
CCAGTCA2100.023.55634727
CTCCAGT2200.023.50767725
TCCAGTC2200.023.50767726
ACTCCAG2300.023.4632424
CAGTCAC1950.023.06215928
GTCACAG1950.023.06215930
AACTCCA2350.022.96402223
GAGTTCA602.389256E-422.4856058
TCACAGA2000.022.48560331
GAACTCC2650.020.36431922
Produced by FastQC (version 0.11.2)