FastQCFastQC Report
Tue 16 Jan 2018
H5TH7BCX2_l01n01_gal-ssb_glu_doxrap-2.351000000b0eea.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TH7BCX2_l01n01_gal-ssb_glu_doxrap-2.351000000b0eea.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9730794
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC6511246.691375852782415TruSeq Adapter, Index 2 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACAC617610.634696408124558TruSeq Adapter, Index 14 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC338800.3481730267848646TruSeq Adapter, Index 2 (100% over 51bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG192870.19820581958676756TruSeq Adapter, Index 2 (100% over 49bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG146490.15054269980435306TruSeq Adapter, Index 2 (100% over 49bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC788200.040.02820645
TCGTATG840200.037.55831544
CTCGTAT851850.036.97865343
TATCTCG828250.036.85411540
TCTCGTA843950.036.51194442
ATCTCGT844000.036.4937941
GTATCTC883900.034.45236239
ATGTATC889100.034.1218437
TGTATCT896550.033.94618238
GTGACTG94800.033.865211
CGATGTA924900.033.5296235
GATGTAT906900.033.48333436
GACTGGA98450.032.999873
CCGATGT942250.032.8958634
TGACTGG99000.032.67982
ACCGATG961850.032.38921433
CACCGAT970750.032.15250432
TCACCGA977600.032.03304731
GTCACCG986050.031.8794330
AGTCACC1005950.031.59534829
Produced by FastQC (version 0.11.2)