FastQCFastQC Report
Tue 16 Jan 2018
H5TH7BCX2_l01n01_gal-ssb_gal_doxrap-3.351000000b0ea7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TH7BCX2_l01n01_gal-ssb_gal_doxrap-3.351000000b0ea7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12419293
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC6453715.196519640852341TruSeq Adapter, Index 7 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC724460.5833343331218612TruSeq Adapter, Index 7 (100% over 51bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACAC598490.48190343846465333TruSeq Adapter, Index 14 (97% over 38bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG135470.10908028339455395TruSeq Adapter, Index 7 (100% over 49bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC864300.036.9305245
TCGTATG900750.035.42066644
CTCGTAT907250.035.13495343
ATCTCGT905400.034.3495441
CATCTCG903450.034.1900740
TCTCGTA913750.034.05547742
GTGACTG59750.032.064231
TCATCTC1012350.030.38110539
ACGACGC102250.030.22251716
GATCATC1012250.030.13745937
CGACGCT102050.030.06135417
CCTACAC103950.029.72825811
AGATCAT1027250.029.70483436
CAGATCA1053000.029.62989635
ACACGAC105200.029.54605514
ATCATCT1040300.029.5259338
CTACACG104550.029.51462612
CCCTACA106800.028.91388910
AGTCACC1126300.028.87765529
CAGTCAC1259950.028.7545128
Produced by FastQC (version 0.11.2)