FastQCFastQC Report
Tue 16 Jan 2018
H5TH7BCX2_l01n01_gal-ssb_gal_doxrap-2.351000000b0e8d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TH7BCX2_l01n01_gal-ssb_gal_doxrap-2.351000000b0e8d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11731792
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC261600322.298409313768943TruSeq Adapter, Index 12 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC1938881.6526716464117333TruSeq Adapter, Index 12 (100% over 51bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG642300.5474866925700694TruSeq Adapter, Index 12 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACAC477580.40708188484760044TruSeq Adapter, Index 14 (97% over 38bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG394880.33658967018849295TruSeq Adapter, Index 12 (100% over 49bp)
GAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTG316890.270112187464626TruSeq Adapter, Index 12 (100% over 51bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCG274020.23357045539164006TruSeq Adapter, Index 12 (100% over 51bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAAACTCGTATGC272800.2325305460580958TruSeq Adapter, Index 12 (98% over 50bp)
GAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTT259090.22084435182621714TruSeq Adapter, Index 12 (100% over 51bp)
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG228290.19459090307772248TruSeq Adapter, Index 12 (100% over 49bp)
AGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGC225320.19205932051983193TruSeq Adapter, Index 12 (100% over 51bp)
GCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCT224570.1914200319951121TruSeq Adapter, Index 12 (100% over 51bp)
ACACTCTTTCCCTACACGACGCTCTTCCGCCAGATCGGAAGAGCACACGTC185950.15850093489553854Illumina Single End PCR Primer 1 (96% over 29bp)
AGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCT180130.15354005594371262TruSeq Adapter, Index 12 (100% over 51bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAACCTCGTATGC160030.13640712348122094TruSeq Adapter, Index 12 (98% over 50bp)
AAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTC158140.13479611639892697TruSeq Adapter, Index 12 (100% over 51bp)
ACACTCTTTCCCTACACGACGCTCTTCCGCCCAGATCGGAAGAGCACACGT139500.11890766559788991Illumina Single End PCR Primer 1 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT262150.039.1788831
CGTATGC3365200.037.7245245
TCGTATG3521150.036.053944
CTCGTAT3539600.035.7515943
TCTCGTA3483150.035.5599542
ATCTCGT3505800.035.32462341
GTGACTG58450.035.244171
AATCTCG3522250.035.00555440
CACTCTT297350.034.6619072
CGACGCT295300.034.6547317
ACACGAC297850.034.56192814
CCTACAC297150.034.53737611
ACGACGC297200.034.4861616
CCCTACA298450.034.46969210
CTACACG298450.034.44722712
TCCCTAC299000.034.353639
TACACGA300700.034.1520813
GACGCTC294800.034.04961418
ACGCTCT291350.033.78906219
CACGACG304450.033.7166415
Produced by FastQC (version 0.11.2)