FastQCFastQC Report
Tue 16 Jan 2018
H5TH7BCX2_l01n01_gal-ssb_gal_doxrap-1.351000000b0e63.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TH7BCX2_l01n01_gal-ssb_gal_doxrap-1.351000000b0e63.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12049509
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC202016316.76552131709267TruSeq Adapter, Index 11 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC1510111.2532543857181233TruSeq Adapter, Index 11 (100% over 51bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACAC818160.6789986214376038TruSeq Adapter, Index 14 (97% over 38bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG373820.31023670757040805TruSeq Adapter, Index 11 (100% over 49bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG271300.22515440255698385TruSeq Adapter, Index 11 (100% over 49bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCG229790.19070486606549694TruSeq Adapter, Index 11 (100% over 51bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACAACTCGTATGC223800.18573370914947654TruSeq Adapter, Index 11 (98% over 50bp)
GAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGTCTT174290.1446448979788305TruSeq Adapter, Index 11 (100% over 51bp)
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG158990.13194728515493867TruSeq Adapter, Index 11 (100% over 49bp)
GAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGTCTTCTG155360.12893471426927022TruSeq Adapter, Index 11 (100% over 51bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACACCTCGTATGC143110.11876832491680782TruSeq Adapter, Index 11 (98% over 50bp)
ACACTCTTTCCCTACACGACGCTCTTCCGCCAGATCGGAAGAGCACACGTC122300.10149791165764514Illumina Single End PCR Primer 1 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC2555450.038.47934345
TCGTATG2651450.037.06739844
CTCGTAT2668300.036.77346843
TCTCGTA2623900.036.5119342
ATCTCGT2639750.036.2772141
CATCTCG2630900.036.20476540
GTGACTG62300.034.805031
ACACTCT139050.033.7546351
ACATCTC2821150.033.68269739
TACATCT2835550.033.51495438
CTACATC2842650.033.4098837
GCTACAT2877300.033.0344536
GGCTACA2984050.032.5981535
CGGCTAC3041700.031.99805634
ACGGCTA3081900.031.70753333
CACGGCT3107000.031.47237632
TCACGGC3132600.031.26674531
GTCACGG3151350.031.1256830
AGTCACG3180900.030.93127629
ACACGAC153450.030.67743914
Produced by FastQC (version 0.11.2)