FastQCFastQC Report
Fri 26 May 2023
H5TC7DRX3_n01_RR23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TC7DRX3_n01_RR23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81170902
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG1316164516.214732959355313No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGAACCCCTAC960029111.827256767455903No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCA866257110.67201520071811No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGG78302549.646626817082801No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT77762079.58004261182166No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGAACCCCTACC70487638.683854467947146No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTG23553862.901761520402964No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGATCCCCTACCAACTG21632132.6650104245484423No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGTACCCCTAC16384532.0185225981596213No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACTCCGAACCCCTACC16022251.9738908408335785No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCTACTGGT15096451.8598351906943205No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCA14718241.8132409074374953No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGGT13558851.6704077034896077No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGG13476261.660232875076342No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGTACCCCTACC12041061.4834207460205382No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGATCCCCTAGCAACTG6250740.7700715214424992No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTAGCAACTG4722550.5818033166614311No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGTTCCCCTACCAACTG3125610.3850653279669111No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACTCCGTACCCCTACC2673620.32938158060631134No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACAGCGAACCCCTACCA2291090.28225508692757906No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCTACTGGT2288070.2818830324196718No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACGAACTGG1821190.22436488385948944No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACTCCGAACCCCTAC1625820.20029591392245463No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGAAGCCCTAC1611930.19858470957979496No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACAGCGAACCGCTACCA1558850.19204542041432532No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACTCCGAACGCCTACC1549370.19087751421069585No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAAGACCGAAGCCCTAC1470520.18116344204232201No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGAACCGCTACCA1287000.15855435485982403No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACGCCTACCTACTGGT1113240.13714766900089395No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAAGACCGAACCCCTAC1092310.1345691587854968No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAAGCCCTACGAACTGG1057730.13030901147310153No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACCTACTGG902910.11123567408429193No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCTTCTGGT875250.1078280490218034No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGTTCCCCTAGCAACTG855090.10534440038623694No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCATCTGGT833850.10272769914519367No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)