FastQCFastQC Report
Fri 26 May 2023
H5TC7DRX3_n01_RR22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TC7DRX3_n01_RR22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98610928
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG1874380719.007839577374224No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT1082024410.972662177968754No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGG1063582310.785643351819992No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCA88086928.932774671788913No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGAACCCCTACC63889896.478986791403078No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGAACCCCTAC49704145.040429190565979No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTG32660943.312101474189554No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGATCCCCTACCAACTG26019142.6385655756124717No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACTCCGAACCCCTACC24048432.438718556628937No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCTACTGGT20776562.1069226729110593No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGGT18407411.866670395800352No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGG17868661.8120364915336769No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCA15152351.5365791913042335No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGAAGCCCTAC14656501.4862957176510903No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAAGACCGAAGCCCTAC12462021.2637564875162721No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAAGACCGAACCCCTAC11049211.120485348236455No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGTACCCCTACC10907211.1060853214970252No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGATCCCCTAGCAACTG8548420.8668836378864623No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGTACCCCTAC7747380.7856512616938358No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTAGCAACTG6032110.6117080654590331No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACTCCGTACCCCTACC4199480.42586355135000853No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGTTCCCCTACCAACTG3578480.36288878652475515No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACGAACTGG3069820.31130626820589297No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCTACTGGT3054680.3097709414112805No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAAGACCGTACCCCTAC2681770.2719546458380353No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACAGCGAACCCCTACCA2674220.27118901061350925No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACTCCGAACCCCTAC2621660.26585897254714No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACTCCGAACGCCTACC2531140.25667946254394847No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAAGACCGTAGCCCTAC2508500.2543835709567605No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGTAGCCCTAC2081880.21112061738228446No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACAGCGAACCGCTACCA1986070.20140465567872967No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACGCCTACCTACTGGT1828480.18542366825713272No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGAACCGCTACCA1751870.17765475242257125No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAAGCCCTACGAACTGG1747570.17721869527482795No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAAGTCCGAAGCCCTAC1651840.16751084626239396No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACTCCGAAGCCCTAC1592390.1614821026732453No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACCTACTGG1320270.13388678382582506No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCTTCTGGT1275230.1293193387248115No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAAGCCCTACCAACTGG1243890.12614119197823592No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCATCTGGT1223980.12412214597554543No Hit
GATCGCGCGGTTCTTCTGGAAAGGACGAAACACCGAACCCCTACCAACTG1138070.1154101297981903No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGTTCCCCTAGCAACTG1135900.11519007305153847No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAAGACCGATGCCCTAC1103810.11193586982570533No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)