FastQCFastQC Report
Fri 26 May 2023
H5TC7DRX3_n01_RR21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TC7DRX3_n01_RR21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73134744
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG1368989718.71873237157978No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT812378811.107973523500677No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGG802982110.979488763917736No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGAACCCCTAC71671309.799897569888259No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCA66878829.144603008386822No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACTCCGAACCCCTACC28816063.940132750037383No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTG23549493.2200140059285642No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGATCCCCTACCAACTG19982002.7322171251464282No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACTCCGAACGCCTACC16418662.24498769011894No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCTACTGGT14855082.031193272516275No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGGT13730081.877367616136046No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGG13376261.8289884217000882No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGTACCCCTAC12378961.6926236864929753No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCA11502381.5727654697198366No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGAACCCCTACC10082071.3785609203745897No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGATCCCCTAGCAACTG6041940.8261381211644085No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACTCCGTACCCCTACC4868520.6656918085335747No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTAGCAACTG4139610.5660250892516969No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACTCCGTACGCCTACC3042100.41595824824381694No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGTTCCCCTACCAACTG2768890.3786011748396904No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACAGCGAACCCCTACCA2336250.3194446130829418No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGAAGCCCTAC2170030.29671670143536705No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCTACTGGT2169690.296670211903661No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACGAACTGG1878500.2568546626757865No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACTCCGAACCCCTAC1801380.24630974301352582No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGAACGCCTACC1768950.24187546209227176No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAAGACCGAAGCCCTAC1624620.22214065588306428No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGTACCCCTACC1562630.2136645203817217No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACAGCGAACCGCTACCA1532900.2095994210357802No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAAGACCGAACCCCTAC1489340.20364329162073772No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACGCCTACCTACTGGT1298480.177546256263644No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGAACCGCTACCA1246910.1704948881751743No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAAGCCCTACGAACTGG1231810.16843020603175968No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACTGCGAACGCCTACC993790.13588479915920673No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACCTACTGG925300.12651989319877843No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCTTCTGGT871090.11910754757000312No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCATCTGGT845940.11566868956292511No Hit
GATCGCGCGGTTCTTCTGGAAAGGACGAAACACCGAACCCCTACCAACTG835660.11426306489840177No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGTTCCCCTAGCAACTG806980.11034153616508181No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAAGCCCTACCAACTGG774760.10593596936635206No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)