FastQCFastQC Report
Fri 26 May 2023
H5TC7DRX3_n01_RR20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TC7DRX3_n01_RR20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79498402
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG958916412.062083964907872No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGAACCCCTAC888328011.174161714596478No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGG819779610.311900357443662No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT814849610.249886532310423No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGAACCCCTACC62738007.891730955799589No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCA40827745.135668010031195No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGATCCCCTACCAACTG40561845.102220796840671No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTG17992002.2631901456333674No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACTCCGAACCCCTACC17760142.2340247795169517No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACAGCGAACCCCTACCA16282102.0481040612615082No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGTACCCCTAC15774291.984227305600432No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGATCCCCTAGCAACTG15377451.9343093210854727No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGGT14834671.8660337348667713No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGG14714591.8509290287369549No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCTACTGGT14651511.842994278048507No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACAGCGAACCGCTACCA11887771.49534703854802No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGTACCCCTACC11094171.3955211326134582No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTAGCAACTG10408221.3092363793677262No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGAACCGCTACCA9054321.1389310693314314No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGTTCCCCTACCAACTG6938490.8727835812347524No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCA6779600.8527970159702077No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACAGCGTACCCCTACCA3561470.4479926527328184No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACTCCGTACCCCTACC3116360.3920028480572478No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGTTCCCCTAGCAACTG2360330.2969028232793912No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCTACTGGT2288370.28785106900639335No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACAGCGTACCGCTACCA2193470.27591372213997456No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACGAACTGG1782610.22423218016382265No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGTACCCCTAGCAACTG1741150.21901698099541675No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGAAGCCCTAC1739590.21882075063596873No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACTCCGAACCCCTAC1704040.21434896263701No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACTCCGAACGCCTACC1616050.20328081563199218No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAAGACCGAAGCCCTAC1483310.18658362466204037No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGTACCGCTACCA1286990.16188878865766385No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACGCCTACCTACTGGT1194530.15025836619961241No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAAGACCGAACCCCTAC1185500.14912249431126928No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAAGCCCTACGAACTGG1115370.140300933344547No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACCTACTGG914300.11500860105338974No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCTTCTGGT831050.1045366924482331No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCATCTGGT822950.10351780404340707No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)