FastQCFastQC Report
Fri 26 May 2023
H5TC7DRX3_n01_RR17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TC7DRX3_n01_RR17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78842654
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG1293758216.409368969238404No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGAACCCCTAC930916211.80726615316628No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCA865667110.979679857047937No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGG77165909.787329076973993No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT75019979.515150263713853No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGAACCCCTACC57159407.249806684589791No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACTCCGAACCCCTACC25840233.277442943511262No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTG23280592.952791264484831No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGATCCCCTACCAACTG17884782.268414252011354No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGTACCCCTAC16195202.0541165445800442No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCA14981221.9001415147694038No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCTACTGGT13830281.7541621569461627No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGG13457561.7068882536602585No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGGT13246561.6801260901237545No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGTACCCCTACC9825121.2461680957619716No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGATCCCCTAGCAACTG4998630.6340007275757105No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACTCCGTACCCCTACC4542510.576148793773482No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTAGCAACTG3602060.4568669136886234No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGTTCCCCTACCAACTG2529420.32081872840049247No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACTCCGAACGCCTACC2270590.2879900516793866No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCTACTGGT2111660.26783218129618014No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACAGCGAACCCCTACCA1979090.2510176788315624No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACGAACTGG1745880.22143851220432026No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGAAGCCCTAC1670310.211853598941507No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACTCCGAACCCCTAC1652180.20955408223574004No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACAGCGAACCGCTACCA1432940.18174679913743136No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAAGACCGAAGCCCTAC1390930.17641846506080325No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGAACCGCTACCA1109430.14071444119575172No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAAGACCGAACCCCTAC1042310.13220128282338137No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACGCCTACCTACTGGT1024660.12996264686878756No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAAGCCCTACGAACTGG1019550.1293145205385907No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACCTACTGG939690.11918548556216792No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)