FastQCFastQC Report
Fri 26 May 2023
H5TC7DRX3_n01_RR15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TC7DRX3_n01_RR15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80892166
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG816501310.093700544500193No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAA77348129.561880194925179No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGAACCCCTAC66463228.216273996174117No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACT65976958.156160634887685No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCA61890637.651004177586245No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGAACCCCTACC50570766.251626393586742No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGG47653955.891046359174014No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT46909355.798997890599196No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGAACCCCTACCAAC42134195.2086860920500015No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACT23717542.9319946754794524No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTG14298811.7676384138360197No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAA13078881.6168290017107467No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGTACCCCTAC11319171.3992912490438196No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACTCCGAACCCCTACC10816751.3371814027083908No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGATCCCCTACCAACTG10626631.3136785087445921No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCA10480851.2956569861165543No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGTACCCCTTCCAACT10264671.2689325193740022No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGAACCCCTTCCAACT9746091.2048249517759235No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGTACCCCTACC8580531.060736833279999No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCTACTGGT8545601.0564187389913628No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGG8113981.0030612853165535No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGGT8091141.000237773333947No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGTACCCCTACCAAC7272950.8990920084894253No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGATCCCCTAGCAACTG3091330.38215443507842284No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTAGCAACTG2117720.2617954376447282No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACTCCGTACCCCTACC1801960.22276075534928808No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCGTACCAA1652310.20426081803768242No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCCAACCCCTACCAA1558370.19264782698487762No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGTTCCCCTACCAACTG1455900.17998034568637958No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACAGCGAACCCCTACCA1314940.16255467803890924No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCTACTGGT1274470.15755172138671622No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACGGAACCCGTACCAA1271610.1571981642820641No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGAAGCCCTAC1223240.15121859884429353No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACTCCGAACCCCTAC1166760.14423646413424016No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACGAACTGG1105110.13661520696577714No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACTCCGAACGCCTACC1096980.13561016526618908No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAAGACCGAAGCCCTAC1062860.13139220428341603No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACAGCGAACCGCTACCA1013280.12526305699367724No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACGGAACCCCTACCAA832000.10285297589880335No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)