FastQCFastQC Report
Fri 26 May 2023
H5TC7DRX3_n01_RR13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TC7DRX3_n01_RR13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119661772
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG1575017213.162241989864565No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGAACCCCTAC96640848.0761665471576No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGG94593557.905076819353803No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT93253847.7931187580942725No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCA86609007.237816936222539No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAA79844126.672483506261298No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGAACCCCTACC71400925.966894757333194No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACT68349375.7118801483233925No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGAACCCCTACCAAC42918873.5866818017704096No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTG28128272.350647957979429No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACT25375022.12056194521338No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGATCCCCTACCAACTG25169512.103387705139449No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCTACTGGT17703541.4794649706507772No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGTACCCCTAC17271401.4433515158040615No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGG16313751.363321779991692No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGGT16310271.3630309602969946No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACTCCGAACCCCTACC15828091.3227357188058355No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCA15356471.2833229646641033No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAA13888491.1606455234508812No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGTACCCCTACC12746141.0651806159113204No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGTACCCCTTCCAACT10820170.904229464360598No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGAACCCCTTCCAACT10100800.8441125207472274No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGTACCCCTACCAAC7676200.641491419665756No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGATCCCCTAGCAACTG7141210.5967829057386849No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTAGCAACTG5143150.42980727378832395No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGTTCCCCTACCAACTG3591310.30012174648391465No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACTCCGTACCCCTACC2752270.23000411526581774No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCTACTGGT2666880.22286816879161708No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACAGCGAACCCCTACCA2260770.18893001183368738No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACGAACTGG2149770.17965386639937106No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCGTACCAA1706250.14258939772344337No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGAAGCCCTAC1659020.13864243962558068No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCCAACCCCTACCAA1611240.13464951864493532No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACTCCGAACCCCTAC1611180.13464450451226814No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACTCCGAACGCCTACC1600600.13376034578528553No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACAGCGAACCGCTACCA1572250.13139116810003448No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAAGACCGAAGCCCTAC1502300.12554552509885947No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACGGAACCCGTACCAA1356500.1133611827175683No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACGCCTACCTACTGGT1325580.11077723301640562No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAAGCCCTACGAACTGG1277840.10678765479087172No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGAACCGCTACCA1209330.10106235097370947No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)