FastQCFastQC Report
Thu 22 Feb 2018
H5T75BCX2_l02n02_lib0208_p90.352000000c5c99.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5T75BCX2_l02n02_lib0208_p90.352000000c5c99.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109661830
Sequences flagged as poor quality0
Sequence length33
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTGATACGCCGCCCGCCACGCCCATCGTGTT3826750.3489591592626167No Hit
GACGTACTCTGCGTTGATACCACTGCTTCCGCG3789160.3455313485102337No Hit
CACGTACTCTGCGTTGATACCACTGCTTCCGCG2330260.2124950860294781No Hit
GGTGCATATGGTGCCAACCCCCCTGGCCCCCCC2086250.19024395270441866No Hit
GTCTAAGACATGTATCACGAATTGAAAATGATA2076550.18935941521311472No Hit
TACGTACTCTGCGTTGATACCACTGCTTCCGCG2002090.18256945009945574No Hit
AACGTACTCTGCGTTGATACCACTGCTTCCGCG1937380.1766685819487054No Hit
GTGCTGGTGCAATTGCAAAGGCACAGGAGCACA1787810.16302937859052688No Hit
GTACTCTGCGTTGATACCACTGCTTCCGCGGAC1574700.14359599871714707No Hit
GGCCAGCGTTACTCACTAAAAAAAAAAAAAAAA1525330.13909397645470625No Hit
GCGTTACTCACTAAAAAAAAAAAAAAAAAAAAA1455190.1326979496876899No Hit
GTGTTGGAGGAACTCAAACAAAACAAGCAAAAA1369450.12487936778001972No Hit
ACGTGTGGGTGGCCCACAAGGACATCTAGTAAC1345120.12266072889719239No Hit
GTCCGGAAGCGGAAACGGAAACGGAAACGGGCG1343380.12250205928535024No Hit
CCCCAAAAGGGGGGTGGTGCTGGTGCAATTGCA1332640.12152268478466938No Hit
CCCCTGGCCCCCCCAAAAGGGGGGTGGTGCTGG1324070.12074119135163074No Hit
GTATCGCGGGCAAGGCGACGGCGCGTCCACAAA1261470.11503273290259701No Hit
CCCAAAAGGGGGGTGGTGCTGGTGCAATTGCAA1234030.11253049488595987No Hit
GTGCCAACCCCCCTGGCCCCCCCAAAAGGGGGG1219630.11121736706381792No Hit
GTGCATATGGTGCCAACCCCCCTGGCCCCCCCA1216080.1108936445798871No Hit
CTCTCGAATTGTGTCCGTTGGTAGCCGAAGTTA1210870.11041854763868157No Hit
GAGCACAGGAGCAGCTAAATAAACAAGAAAGCA1191270.10863123476965504No Hit
CTAGTAACACGACGCCCAACAGCAGCCGCAAGG1187810.10831571933461259No Hit
ACTTAAATGTGACCTAGTAGATAAAATCATCAT1161970.10595938440932455No Hit
GCACAGGAGCAGCTAAATAAACAAGAAAGCAAA1151730.10502560462469028No Hit
CCATAGAGACGTTTAAGGCAAATTCTAAATGAA1150580.10492073677778312No Hit
GGCACAGGAGCACAGGAGCAGCTAAATAAACAA1106060.10086098326099427No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA423150.020.156571
TTCCGCG1605050.018.0155727
CTTCCGC1895050.015.29212326
GTATCAA1482950.015.074491
TGTTGGA315350.014.2426552
GTGTTGG335300.013.9160721
GTTGGAG328700.013.3488923
GACCTAG258150.013.19288111
CGCGGAC360050.012.9641927
GGAACTC322150.012.8624929
CCCGCCA852800.012.84508914
CGCCCGC859800.012.81755512
CCGCCCG854700.012.78740911
ACGGGCG319000.012.48063227
GCCCGCC893750.012.46574913
CCGCCAC885300.012.41341515
TTGGAGG357500.012.24784854
TGACCTA294650.012.004209510
GTCTGAT932300.011.9796061
TCTGATA928500.011.9705792
Produced by FastQC (version 0.11.2)