FastQCFastQC Report
Thu 22 Feb 2018
H5T75BCX2_l02n01_lib0208_p90.351000000c5c9c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5T75BCX2_l02n01_lib0208_p90.351000000c5c9c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109661830
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTCCGAGCC4066320.3708054115091824No Hit
GCTGTCTCTTATACACATCTCCGAGCC2458210.2241627738658018No Hit
ACTGTCTCTTATACACATCTCCGAGCC2114980.192863825088456No Hit
TCTGTCTCTTATACACATCTCCGAGCC2039190.1859525780301131No Hit
GTCTCTTATACACATCTCCGAGCCCAC1620580.14777976986158264No Hit
CTCTTATACACATCTCCGAGCCCACGA1291300.1177529136619369No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTTTT11416600.019.86643219
ATTTTTT21980400.019.85382520
GGATTTT6500600.019.70032718
GCTTTTT12750600.019.64320619
CTTTTTT25650050.019.56226220
GGCTTTT6845750.019.50045618
TATTTTT4842800.019.48913219
AGTTTTT4440350.019.46341719
TGTTTTT10810500.019.44372619
GTTTTTT48416100.019.43615220
GTATTTT2358400.019.39875218
CGTTTTT5543500.019.34290319
GGGCTTT3718100.019.338517
TGGTTTT6389850.019.28668818
GGGATTT3635700.019.28219617
GTGTTTT5448850.019.22816518
GAGTTTT2389400.019.13433618
AGGTTTT3211950.019.12876918
GGTTTTT28347850.019.12340519
TCTTTTT6411200.019.12329519
Produced by FastQC (version 0.11.2)