FastQCFastQC Report
Thu 22 Feb 2018
H5T75BCX2_l01n02_lib0202_p70.352000000c5c8c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5T75BCX2_l01n02_lib0202_p70.352000000c5c8c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106523271
Sequences flagged as poor quality0
Sequence length33
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACGTACTCTGCGTTGATACCACTGCTTCCGCG4771940.4479715986190473No Hit
GTCTGATACGCCGCCCGCCACGCCCATCGTGTT3849100.3613388852845122No Hit
CACGTACTCTGCGTTGATACCACTGCTTCCGCG3443780.3232889834935692No Hit
GGTGCATATGGTGCCAACCCCCCTGGCCCCCCC3341600.31369671327498005No Hit
GTGCTGGTGCAATTGCAAAGGCACAGGAGCACA2610070.24502345595452096No Hit
GTCTAAGACATGTATCACGAATTGAAAATGATA2542090.23864175181026875No Hit
TACGTACTCTGCGTTGATACCACTGCTTCCGCG2529500.23745985043962833No Hit
AACGTACTCTGCGTTGATACCACTGCTTCCGCG2353940.22097894459136538No Hit
GCGTTACTCACTAAAAAAAAAAAAAAAAAAAAA2276140.21367537615325388No Hit
GGCCAGCGTTACTCACTAAAAAAAAAAAAAAAA2249580.2111820242545875No Hit
CCCCTGGCCCCCCCAAAAGGGGGGTGGTGCTGG1971320.18506003256321335No Hit
CTCTCGAATTGTGTCCGTTGGTAGCCGAAGTTA1942680.1823714181664587No Hit
GTACTCTGCGTTGATACCACTGCTTCCGCGGAC1939680.18208978956344665No Hit
GTGCCAACCCCCCTGGCCCCCCCAAAAGGGGGG1931770.18134722881350498No Hit
GTGCATATGGTGCCAACCCCCCTGGCCCCCCCA1890210.17744573389977858No Hit
CCCCAAAAGGGGGGTGGTGCTGGTGCAATTGCA1826920.17150430913823517No Hit
ACGTGTGGGTGGCCCACAAGGACATCTAGTAAC1711860.16070291345071444No Hit
CCCAAAAGGGGGGTGGTGCTGGTGCAATTGCAA1650910.15498115899952042No Hit
GAGCACAGGAGCAGCTAAATAAACAAGAAAGCA1632820.15328293852335798No Hit
GCACAGGAGCAGCTAAATAAACAAGAAAGCAAA1605620.15072950585604905No Hit
CTCTGAAGCAAAAGCCAAGGCCGGAGCAGGTGC1518100.14251346074417862No Hit
CTCTCTCACTCTCTGAAGCAAAAGCCAAGGCCG1499820.14079740378982541No Hit
ACTTAAATGTGACCTAGTAGATAAAATCATCAT1464980.1375267569468459No Hit
GCCCACAAGGACATCTAGTAACACGACGCCCAA1452690.1363730184365067No Hit
GGCACAGGAGCACAGGAGCAGCTAAATAAACAA1433850.13460439080959125No Hit
GTATCGCGGGCAAGGCGACGGCGCGTCCACAAA1396760.13112252251435275No Hit
CTAGTAACACGACGCCCAACAGCAGCCGCAAGG1358360.127517676395799No Hit
GTACGTACTCTGCGTTGATACCACTGCTTCCGC1356280.12732241389771065No Hit
GTGTTGGAGGAACTCAAACAAAACAAGCAAAAA1354380.12714404911580307No Hit
GTCCGGAAGCGGAAACGGAAACGGAAACGGGCG1353930.12710180482535124No Hit
GTCGTGCATTCCGCAGCAACGACGGTTGGGCAC1325690.12445074090899819No Hit
CATTAGCAGCGGGCAGATGGACAACGTGTGGGT1323140.12421135659643798No Hit
AGTCTAAGACATGTATCACGAATTGAAAATGAT1292570.12134156113174557No Hit
GAGTGAATGGGCCAGCGTTACTCACTAAAAAAA1286020.12072667201516935No Hit
GAGCAGGTGCATATGGTGCCAACCCCCCTGGCC1282570.12040279912170554No Hit
TCCCCACTCTCTCGAATTGTGTCCGTTGGTAGC1271730.11938518110282212No Hit
CATCCCGTCTCATCCCCACTCTCTCGAATTGTG1254900.11780524463992474No Hit
CTCGAATTGTGTCCGTTGGTAGCCGAAGTTACG1250600.1174015769756075No Hit
GTGAATGGGCCAGCGTTACTCACTAAAAAAAAA1243160.11670313804013772No Hit
CGTGTGGGTGGCCCACAAGGACATCTAGTAACA1219920.11452145512880468No Hit
GCAGAGGAAGCGGTCCGGAAGCGGAAACGGAAA1218520.11439002844739905No Hit
CCATAGAGACGTTTAAGGCAAATTCTAAATGAA1191100.11181594301586928No Hit
TAGTAACACGACGCCCAACAGCAGCCGCAAGGT1156100.10853027598072913No Hit
CTCTCACTCTCTGAAGCAAAAGCCAAGGCCGGA1102020.10345345103043259No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA548550.022.388591
TTCCGCG2057750.018.44765327
GTATCAA2026000.015.9900831
CTTCCGC2448000.015.48891926
TGTTGGA294350.014.6649162
GTTGGAG315500.013.686673
GTGTTGG336200.013.5630991
GGAACTC296450.013.5556559
GACCTAG316350.013.41505111
CGCGGAC429750.012.94492827
CCCGCCA838700.012.90582314
CGCCCGC843850.012.87778512
CCGCCCG850750.012.69924211
ACGGGCG299850.012.66351227
TTGGAGG333550.012.6541644
GAACTCA316750.012.60171810
TGACCTA344850.012.59583810
CCGCCAC874250.012.38260115
GTGACCT355500.012.2830039
CCTAGTA349400.012.21376713
Produced by FastQC (version 0.11.2)