FastQCFastQC Report
Thu 22 Feb 2018
H5T75BCX2_l01n01_lib0202_p70.351000000c5c8f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5T75BCX2_l01n01_lib0202_p70.351000000c5c8f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106523271
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTCCGAGCC5120770.480718433815274No Hit
GCTGTCTCTTATACACATCTCCGAGCC3632960.3410484831995067No Hit
ACTGTCTCTTATACACATCTCCGAGCC2669130.25056778438581745No Hit
TCTGTCTCTTATACACATCTCCGAGCC2481900.23299134327183774No Hit
GTCTCTTATACACATCTCCGAGCCCAC1993910.1871806959438938No Hit
CTCTTATACACATCTCCGAGCCCACGA1623920.15244744033442234No Hit
ACCTGTCTCTTATACACATCTCCGAGC1443140.13547650071691847No Hit
CTGTCTCTTATACACATCTCCGAGCCC1098540.10312676185093865No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTTTTT19530000.019.92549320
GATTTTT10640800.019.87558619
GGATTTT6071850.019.76067718
GCTTTTT12297250.019.68765619
CTTTTTT24406700.019.5698520
GGCTTTT6685500.019.48681618
AGTTTTT3734050.019.47128519
TATTTTT3933150.019.44280219
GTTTTTT44733700.019.44147120
GGGATTT3386700.019.43407217
CGTTTTT5357000.019.40378619
TGGTTTT5778250.019.3863218
CATTTTT3358100.019.32431619
TGTTTTT10010700.019.29636619
TGATTTT2177150.019.26834518
GGGCTTT3643650.019.21660217
AGGTTTT3014900.019.20974718
GTATTTT2055650.019.19342218
GTCTTTT3010050.019.1915518
TCTTTTT5863300.019.18832619
Produced by FastQC (version 0.11.2)