FastQCFastQC Report
Wed 17 May 2023
H5NY3DRX3_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5NY3DRX3_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences961717824
Sequences flagged as poor quality0
Sequence length90
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGTTGACCTCAAATCAGGTAGG55345220.5754829391619969Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12379100.12871862921821026No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGACCACATCGATTCAATC12378760.12871509387768196Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGCATATCAATAAGCGGAGGAA11338260.11789591205496883Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)