FastQCFastQC Report
Wed 17 May 2023
H5NY3DRX3_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5NY3DRX3_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences961717824
Sequences flagged as poor quality0
Sequence length10
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCCGAGGTG25698466126.721420211506864No Hit
GTGCACGGAA20913911821.746411762458923No Hit
TCGTTGTATT20485727721.301183350013485No Hit
TTCGACAAGC18581643719.32130531044416No Hit
AGATCTCGGT500463285.203847402125303Illumina Single End PCR Primer 1 (100% over 10bp)
GTTTGGTGTC85575670.8898209834987939No Hit
GTTACGGGCT83897950.8723759496423766No Hit
GCAGACACCT77285100.8036151360755065No Hit
CTCCTGCCAC58335400.6065750113413725No Hit
GGGGGGGGGG40355990.4196240206108523No Hit
GCACGGAAGT10156170.1056044688634158No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)