FastQCFastQC Report
Wed 17 May 2023
H5NY3DRX3_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5NY3DRX3_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences961717824
Sequences flagged as poor quality0
Sequence length10
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAATACGCG25366329026.3760620495685No Hit
GCAGTATAGG20483670421.299044157052037No Hit
CCTTCTAGAG20161882320.964446947798276No Hit
TGCAATGTTC18359351819.090164850682857No Hit
GGGGGGGGGG554010035.760629741640309No Hit
CCCAGCTTCT84038620.8738386447956693No Hit
CCTGTCAGGG83095210.8640290106550007No Hit
CGCTGAAATC75281240.782778878807595No Hit
TGGTTCGGGT55951750.5817896747227178No Hit
TAATACGCGA11350430.11802245644976213No Hit
CCTCTAGAGA10258570.10666923024606435No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)