FastQCFastQC Report
Wed 31 May 2023
H5NT3BGXT_n01_DEL2_LerAmpPoolHm-B_aa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5NT3BGXT_n01_DEL2_LerAmpPoolHm-B_aa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2099
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAAAACTTTAATTAAATATTAAATATAAACGATTAGTTAACTAAAATA70.3334921391138637No Hit
CTTACACATGCATGGCTTAATCTTTGAGACAAGCATATGACTACTGGCAG60.28585040495474034No Hit
AATGAAGAAAACATGAGATATTTTCCTTTTTTTAATTTCTTGTCAATACC50.23820867079561697No Hit
CAAGACATAAAGGATCGTAGGATGCTTTTGTAGGACATTTGTTTCAAAAA40.19056693663649357No Hit
GGTCCCTGCGGATGCTAACGCAATGTGATTTCTGCCCAGTGCTCTGAATG40.19056693663649357No Hit
GAAGTGATTCAGTCCCGCCAAAGCTCATCCGTCCTTCGGGCAAAAGATGA40.19056693663649357No Hit
AGACACATGGATATGTCAGTGTGGATTTGTAAAATATTATGAATTCTTTA40.19056693663649357No Hit
ATATTCGACTCTAAAACACTAACCAACCTTCTTCTTGCTTCTCAAATCTT30.14292520247737017No Hit
GATTATACAAGTCAATATATATTCAGAACTAATTGTAACTCATTTATTAA30.14292520247737017No Hit
TTTTTAAAATCTCATGTAAATACTTTAAAACTCTTTTAAAATCTCTTGTA30.14292520247737017No Hit
ATATGATAATGTAAACAAGATTAAATTTCAAATCAAGATGTCAAGAGTCT30.14292520247737017No Hit
CTTTAATGCAACCCTAAAGCTTCAATTTTTGAGTGTGTATTTCACTCTCT30.14292520247737017No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)