FastQCFastQC Report
Mon 12 Jun 2023
H5NT3BGXT_n01_DEL2_LerAmpPoolHm-B_aa_a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5NT3BGXT_n01_DEL2_LerAmpPoolHm-B_aa_a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1088
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAAAACTTTAATTAAATATTAAATATAAACGATTAGTTAACTAAAATA70.6433823529411764No Hit
CTTACACATGCATGGCTTAATCTTTGAGACAAGCATATGACTACTGGCAG60.5514705882352942No Hit
AATGAAGAAAACATGAGATATTTTCCTTTTTTTAATTTCTTGTCAATACC50.4595588235294118No Hit
CAAGACATAAAGGATCGTAGGATGCTTTTGTAGGACATTTGTTTCAAAAA40.3676470588235294No Hit
AGACACATGGATATGTCAGTGTGGATTTGTAAAATATTATGAATTCTTTA40.3676470588235294No Hit
GAAGTGATTCAGTCCCGCCAAAGCTCATCCGTCCTTCGGGCAAAAGATGA40.3676470588235294No Hit
ATATTCGACTCTAAAACACTAACCAACCTTCTTCTTGCTTCTCAAATCTT30.2757352941176471No Hit
ATATGATAATGTAAACAAGATTAAATTTCAAATCAAGATGTCAAGAGTCT30.2757352941176471No Hit
GATTATACAAGTCAATATATATTCAGAACTAATTGTAACTCATTTATTAA30.2757352941176471No Hit
TTTTTAAAATCTCATGTAAATACTTTAAAACTCTTTTAAAATCTCTTGTA30.2757352941176471No Hit
GGTCCCTGCGGATGCTAACGCAATGTGATTTCTGCCCAGTGCTCTGAATG30.2757352941176471No Hit
CTTTAATGCAACCCTAAAGCTTCAATTTTTGAGTGTGTATTTCACTCTCT30.2757352941176471No Hit
ACTAAAGATTTCCATAATATTCAACCAAAAACCAAAGTTTTCCTTATTAG20.1838235294117647No Hit
ATATGAGTCTTTGGCTTTGTGTCTTCTAACAAGGAAACACTACTTAGGCT20.1838235294117647No Hit
CTATATAGGGGTGGGTGTGTGTAGTGGGAATTTTGCCCGCACGCGCGCGC20.1838235294117647No Hit
ATCAGAAAGAGTGGAGAATCTAATTGGCTATCACATTTTAGGGTTTAGGA20.1838235294117647No Hit
TGTAATGCATTGAAAATCAACTGAAAATTTGTTATTGTCTAATGCATTAC20.1838235294117647No Hit
CAAACATATTGGGGGGAAAATATGATTTTACGGTTTTGGCGGGAAAACAT20.1838235294117647No Hit
AGATTAGATTTTGCGACCTAATTGTTTTTGTGACTACCATTGAAACTGCA20.1838235294117647No Hit
CCATCTTTTTTTTAACATATCATATTTCCAATATTCTTGAATGAGAATAT20.1838235294117647No Hit
ATTTAAAAATGACACCAAACTAATCAAACTATGGTTTATAGACTGACCTT20.1838235294117647No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)