FastQCFastQC Report
Wed 31 May 2023
H5NT3BGXT_n01_DEL2_An1AmpPoolHm-B_aa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5NT3BGXT_n01_DEL2_An1AmpPoolHm-B_aa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4842
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAAAAAATCTATTTCCCGCCAAAAAAATTTTTTTTGCGGAAAAAATA250.5163155720776538No Hit
GAAATATTTTTTTTGGCGGGAAACTTTTTTTTTGGCGGGAAAAAAGTTTG110.22717885171416768No Hit
AATTTGTTTTCAAACATATTTTTCCCGCCAACTTTTTTTTTTTGGCGGGA100.20652622883106153No Hit
GAATCCTTTCCCGCTAAATTACACTTCAGTCACCGATAATAATCTCCCGC90.18587360594795538No Hit
CACAGGTTTACGTTTTTTGGCGGGAAAATTATGGGTTTACTTTTTGGGGA90.18587360594795538No Hit
AAAGTGGGATCAGTGTACAAGAAATTGAATTTCCGCTAGGGTTGGCGGAA70.1445683601817431No Hit
AAACATGATGATAGAAAAAAAATTGGAAAGAGGTTTTAAAGTTCCTAACA70.1445683601817431No Hit
CATATTTTTCCGCCAAAACTGTAAAATCATATTTTTCTGCCAAAATCGTA70.1445683601817431No Hit
AAATAAATTCATGACATCAAAAATAAATAAACCAAATCGAGTTCGAAGAG60.12391573729863693No Hit
AAGGAGATCCGCCGGATTCATCATCGGTAATTCCCCGACATTCCCTTTCA60.12391573729863693No Hit
AAAAAAGATCTTTTACTTGTGTTTTTGTTTTGACGAGACAAAATAAAAAA60.12391573729863693No Hit
ATAATATAAAATTATTTTTTCCCAATTTTGAAAACAGTGTTACAAACTTT50.10326311441553077No Hit
GGAAACTATTATTAATTTTTAATCATTGCAAATTTCATAGTTATTTGCCA50.10326311441553077No Hit
AGTTATTGTCTCTGCTTTGCAACACGTGATCTCAGGGGAAAACGAAACGG50.10326311441553077No Hit
CCAAAGACTCATATGGACTTTGGCTACACCATGAAAGCTTTGAGAAGCAA50.10326311441553077No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)