FastQCFastQC Report
Mon 12 Jun 2023
H5NT3BGXT_n01_DEL2_An1AmpPoolHm-B_aa_a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5NT3BGXT_n01_DEL2_An1AmpPoolHm-B_aa_a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1616
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAAAAAATCTATTTCCCGCCAAAAAAATTTTTTTTGCGGAAAAAATA231.4232673267326734No Hit
GAAATATTTTTTTTGGCGGGAAACTTTTTTTTTGGCGGGAAAAAAGTTTG110.6806930693069307No Hit
GAATCCTTTCCCGCTAAATTACACTTCAGTCACCGATAATAATCTCCCGC90.5569306930693069No Hit
CACAGGTTTACGTTTTTTGGCGGGAAAATTATGGGTTTACTTTTTGGGGA80.49504950495049505No Hit
AAACATGATGATAGAAAAAAAATTGGAAAGAGGTTTTAAAGTTCCTAACA70.4331683168316832No Hit
CATATTTTTCCGCCAAAACTGTAAAATCATATTTTTCTGCCAAAATCGTA70.4331683168316832No Hit
AAAAAAGATCTTTTACTTGTGTTTTTGTTTTGACGAGACAAAATAAAAAA60.3712871287128713No Hit
GGAAACTATTATTAATTTTTAATCATTGCAAATTTCATAGTTATTTGCCA50.3094059405940594No Hit
AAATAAATTCATGACATCAAAAATAAATAAACCAAATCGAGTTCGAAGAG50.3094059405940594No Hit
ATTAGCAAACATTTGAAGATTAATCTACATGGGCTGTGATCATGCTAATA40.24752475247524752No Hit
AAATATACTATAAAATAGATAAATGATTATAGAAAAAACTATGAGTATTG40.24752475247524752No Hit
CAAAAATATTGTATATATATGAGCATTTGGCATTTCAGCATTGTAATTAA40.24752475247524752No Hit
ATAATATAAAATTATTTTTTCCCAATTTTGAAAACAGTGTTACAAACTTT40.24752475247524752No Hit
CATGCATGGCTTAATCTTTGAGACAAGCATATGACTACTGGCAGGATCAA40.24752475247524752No Hit
CCAAAGACTCATATGGACTTTGGCTACACCATGAAAGCTTTGAGAAGCAA40.24752475247524752No Hit
AGTTATTGTCTCTGCTTTGCAACACGTGATCTCAGGGGAAAACGAAACGG40.24752475247524752No Hit
ATAGAAATCCTCAAGCAAAGCTAGACACTGACCTAAATCCCTTTAGCGAT40.24752475247524752No Hit
TAATATTTTTAAAATTACATATTGAAACAAAAAAAACCGCAAAATCATAT30.18564356435643564No Hit
CTCCACCATTGTAATATACCATCTCCTGTTTTTATTTTGTTTCTGTGATG30.18564356435643564No Hit
CTTGTTCTTGTGGAATAGATGGACTTTGCTTTTATAGGCAATTTTGGTCC30.18564356435643564No Hit
AATTATTTTGCCTCCAAAATACACCTTTACCCGCTATAATACTGATTTAT30.18564356435643564No Hit
AAAAAAATTTCCCGCCAAAAATTTTCGAAAAAAAAATTCCCGCCAAAAAT30.18564356435643564No Hit
GTAGTGTATCCTTGTTAGAAGACACAAAGCCAAAAACGCAAATCCGTAAT30.18564356435643564No Hit
AGGACGGTGGTCATGGAAGTCGAAATCCGCTAAGGAGTGTGTAACAACTC30.18564356435643564No Hit
CGATTTTATAATCTGAATAAAGAAGAAAAAGAATCGGCCGGTCAAGTAGA30.18564356435643564No Hit
TTTCAAGTCCTCGAACATTCTTTTGGATGAGGACTGGAAAGCAAAGCTCT30.18564356435643564No Hit
GTAGTTACCATCAAACAAACTATAACTGATTTAATGAGCCATTCGCAGTT30.18564356435643564No Hit
ATTCTTAAATAAATTGTTGCAAGTGTTTAGTTTAACCATTGTAACATTTG30.18564356435643564No Hit
AGACAAATCTCTTATGAACAAGTCTCACATTTCACTGTTACGAAGCAGTA30.18564356435643564No Hit
GACCAGGCCAGGCTATTAAAATAAAAAAGATCTTTTACTTGTGTTTTTGT30.18564356435643564No Hit
GAAACAATTCTAAGTCATAAAATTAAGCTGTAGCGTTATGTATTAATTTG30.18564356435643564No Hit
CAATAGATATTTAGATGGATAAGTAGATCGAAAACCGCTTAGGATCAATT30.18564356435643564No Hit
CCTCAATTTACACAATGGTTGTCAGGTAAAAAACAACGAGAGTTGATAAG30.18564356435643564No Hit
AAAAGGAGAAACCTAGACAGTGAAATCAACAGAATTGAACGAAAACATCA30.18564356435643564No Hit
ATACAAAGCCAAAGTCGAATATGGACTTTGGCTAGACCATGAAAGCTTTG30.18564356435643564No Hit
AAAGAAAAATTACAGGTTTACGTTTTTGGCGGGAAAATTACGAGTTTACG30.18564356435643564No Hit
TTATCCGCTCCAACAACTTGATAACGGGTCGGGTCTCCCAGCAAAAGCTT30.18564356435643564No Hit
AACTGAAATGTTTTTTTTTGCGGAAAAAATAAATTTTGATGTCAAAAATT30.18564356435643564No Hit
TCCAGATATGAGTTCATCAAGATCACACTCGTAAAGGAAATGACTTTATG30.18564356435643564No Hit
ATAATAGGATCCACCAATTCTTCTGCGAGAGCTACTGTACCCGAACAGTA30.18564356435643564No Hit
ACAAATGGGTGAAAATGAGGAAGCTTTGTGGTTGTTTAAAGAAGCTTTGT30.18564356435643564No Hit
AAAAAAAGAAGTGGAGCCCATGTTTTCACCTACACAGATCAAACATCAAG30.18564356435643564No Hit
GAAACACTTGTGTAGAATTGGGGATTGTTTTTTTTGGAGTGATTTAGGGG30.18564356435643564No Hit
CATTGGACAGGATGGCGGAATAAACCAGATTATTATCTATTCTTATTTTT20.12376237623762376No Hit
GTTGACTTCGATAGTAGGTTTCGATCTTTTTGCAATGACAATATTGTTAT20.12376237623762376No Hit
CCAGGAAAAAAAAGTGAATTTTCCCGGAAAGATGAGAAATTTGAGAAACG20.12376237623762376No Hit
ACATGTCTTGAGTGTAACTCAGCCGTAACAATGTTACGGCTGAGTTGTCA20.12376237623762376No Hit
CATCGCATTTGTTGCCTTGCTCGCATTGGTGAATGAGTTGCGGGTTGAAA20.12376237623762376No Hit
CTAATAAAGAGACCTGCGATCCCATAGAAAGACATCACAATCCCTTGTGG20.12376237623762376No Hit
ATATCTTAATGATGTTGACACTAAAGATAGAAGAAGAAGATAAAATCAGC20.12376237623762376No Hit
ATTTTCCGGCCAAAAAAACGTAAACCCGTGATTTTCTCGCCAAAAACGTA20.12376237623762376No Hit
CATAACAATGACAATTGAAGATACTTTCTAATATTGTTCTTTCTTCTCTG20.12376237623762376No Hit
AATTACTCGTACCTACATTCATGTTGAAATCTTCCCCATGCATATACCAA20.12376237623762376No Hit
CTCCGCTTATTGATATGCTTAAACTCAGCGGGTAATCCCGCCTGACCTGG20.12376237623762376No Hit
ATTGTCCAAACTTAAGGAGTCTGCATTGTTTGTTGGTGTAAGAAAAGAAA20.12376237623762376No Hit
AACCGAGCTTCAATATTATGACAATAAAAAAGGTTTGGTGAGTTTTCAAT20.12376237623762376No Hit
CTCGGATTTTCGTTTTTGGCGGGAAAATCTCGGGTTTACGTTTTTGACGA20.12376237623762376No Hit
ATAATTTCTAACAATATTATCGTCATTGAAATCTTCTAATGTAAAAAATA20.12376237623762376No Hit
ATGCTACCTACACAGAACTTTAATTGTAGGTTTAGCTTATAAAAATACAA20.12376237623762376No Hit
TTCAACATTTTCCCGCCAAAACGCAATTTAACATTTTCCTGCCAAAAACG20.12376237623762376No Hit
CATAACAACAAGTCATATTCGACTCCAAAACACTAACCAACCTTCTTCTT20.12376237623762376No Hit
CCTTTTTTTTTAGAGGATTTTAATATCTAATAAAAGGCGCCAAAACTCAA20.12376237623762376No Hit
TACTTGTCTATCTCAAAACGCCAAAGTCCAAACTCAATTCATTACATTGA20.12376237623762376No Hit
CAACCCGATCTTAAAAGCCTAAGTAGTCTTTCCTTGTTAGAAGACACAAA20.12376237623762376No Hit
ACATGTTTTGGATTCTATCAAAACACGTGTCAATTTGAGAGTACAACCAA20.12376237623762376No Hit
ATAAACCCGTGATTTTTCCGCCAAAAACGTAAACCCGTGATTTTTCCGCC20.12376237623762376No Hit
CTTATCAAATTCAAATCCTAACATAACAAATCCGGAGTCAAATCCAAACA20.12376237623762376No Hit
CTTCCTCCCAAGTGAGCTTGTTTAAAGTCATTAGCTTGACTCCTTTACTC20.12376237623762376No Hit
CGATAACCTCACGCAAGAAGAACTCAAGAAAATCGCCGCTTATAAAGCCG20.12376237623762376No Hit
CATTGTACCGCCTACCGATGAAAATATACCGAACCAATCGAAAACTGGTC20.12376237623762376No Hit
CCCTACTTTAGATTCTGGAATCCAGTTGCGGTTCTAGTTCTTATACTCTT20.12376237623762376No Hit
GGAAGGCAACAGGCGCGCAAATTACCCAATCCTGACACGGGGAGGTAGTG20.12376237623762376No Hit
ATATTATTGGCATCACTCTCTGAAGCCACACAACATGAGTCTTCCTTCAA20.12376237623762376No Hit
AAATCGCCGGCGCTTTCCAATCAACCGGAGCAAACTCTCCCGTCCGGTGA20.12376237623762376No Hit
CCATTTGCTTTCATATTGTAAATTACTCCCTTCATTTGAGATTACAGTAA20.12376237623762376No Hit
ATTAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20.12376237623762376No Hit
CAATCAACATTTTCCCGCCAAAACGCAATTCAACATTTTCCCGCCAAAAA20.12376237623762376No Hit
GAAAATGCTGAATGTCGTTTTTGGCGGGAAAATATTAAATTGCGTTTTGG20.12376237623762376No Hit
TGATAGAGTTTGATTATTACCGCAATTAGAAGTATTTCTAGAGTCCACTT20.12376237623762376No Hit
AAATTTGGCGGGAATTTTTTTTTTTTGCGGGAAAAAAAAATTTTGGCGCA20.12376237623762376No Hit
CTCAAAGCTTTCATGGTGTAGCCGAAGTCCATATGAGTCTTTGGCTTTGT20.12376237623762376No Hit
CTTTACGCAATTGATCGGATCATCTAGATATCCCTTCAACACAACATAGG20.12376237623762376No Hit
CAAGTATTTGGATGGATCATCTGGATGATACATCTGAATGAGTTTCTAAA20.12376237623762376No Hit
AGAGAGAGAACAAGAGAGACTCTAGCTGATATAGAGGTTTTTACTCGAAA20.12376237623762376No Hit
GTATCCTTGTTAAAAGACACAAAACCAAAGACTCATATGGACTTTGGCTA20.12376237623762376No Hit
GATATGCCATAAAAACTAATGAAATCGTGACGTCCTCAACTTGCAATTGT20.12376237623762376No Hit
AACCATCATTGATCGAATGAACCACTATATGATCAAACAACCATACACTT20.12376237623762376No Hit
CATTTTTCGTTTTACTGCAAATTTCAGAGCAGTTTTTTTTTCACTCATAT20.12376237623762376No Hit
AAAGATTTCGTTGAATTTACGCGGTAACCCGAGAATCAAGGTGTGTCGTC20.12376237623762376No Hit
CAAAATCATATTTTCCCGACAACACCGTAAAATCATGTTTTTCCGCTAAA20.12376237623762376No Hit
AATATTCAAAAATAAAATTTGGCGGGAATTTTTTTTTTCGAAAATTTTTG20.12376237623762376No Hit
ATCGATTCTTTTGGATCCTTCCTTTATTCAAACGGAAGGAAGAGAGATAG20.12376237623762376No Hit
AATTAGATGACGAGGCATTTGGCTACCTTAAGAGAGTCATAGTTACTCCC20.12376237623762376No Hit
TTATAGTTCTCCCTTTGCTCTTGTGAATTGCATATGTTGTGATATAAGTT20.12376237623762376No Hit
ATTATGAATATCACATCTCAACTGGGTATGTATATTTCTAGGCTTCCACA20.12376237623762376No Hit
ATATCTCCCTCATGCATCCATTTTTTTCTGCTTTTTTGGCGCCAAAAGAT20.12376237623762376No Hit
ATAACAACTTAAACCGCAACTCGATCTTAAAAGCCTAATTAGTGTATCCT20.12376237623762376No Hit
GGGCTTGCCCCAAAAGTAAAAGAAATTTTGAGTTTTGGCGCCTTTTATTA20.12376237623762376No Hit
AGAACTCTACGCGAGAAGAGCGGAAGAATATCATCTATGGGTTAAAGAAT20.12376237623762376No Hit
GCAATGAATCCAATAAAAACATCAAAACTCATATTTGATTGTTTAATCCT20.12376237623762376No Hit
AAAGTTGTCTCATATTTGCTTTGCGGATGACTTGATCTATTTACGGAAGG20.12376237623762376No Hit
AAAAAGAAACTCGGAGCTTCGAACATGAGTTACTTTTGTCATTGAATTAG20.12376237623762376No Hit
CTTTTAGTTCAAAATGATCAATATGTGGAATCAGAACAAGTGATTGCTGA20.12376237623762376No Hit
ACATGATTTTACGGTCTTGGCGGGAAAACATGAGTTTGTAGTTTTGGCGG20.12376237623762376No Hit
AGCAACATATCCACTTAGGAAAACTATCACAGATTTATGCTTTTTCAAAT20.12376237623762376No Hit
CAGAATTCTATCACAATGAAATTGTGTAGACAATTCGTCATCAAGATTAG20.12376237623762376No Hit
AGGTATTTGTTTTTTTTCTTTTCGTTTCTTATGGTGCTCATTGGATCAAC20.12376237623762376No Hit
CTCTGGTACTGCATGGTTCCGCTCTGCAAGAACTCCGAATCATTCTCTTG20.12376237623762376No Hit
CTAAAAGCAGCTTGCTTTACGTTTTACATGGTAACCGAAATGCTTCTTTA20.12376237623762376No Hit
AACGTAATTCATCGATCTGTGAACTCCGATCTGAGAATTTGAAAGTGTCT20.12376237623762376No Hit
CTAAAAAAGGTGGCAAAACAATAAACAAATGTACAATATACACTCAACAA20.12376237623762376No Hit
CTATCTTAAAAGTTAAAGCTAAGATAACACTAAGAAATCACGATACTTCT20.12376237623762376No Hit
AGCAATAAAGGAGTGTTGTAATTGACTGATCATCAGTCAACAAACATAAA20.12376237623762376No Hit
ATCAATCATGGACAACATTGCCGCGCAGTTGAAGCGTGGGATATCGAGAC20.12376237623762376No Hit
CTTTTCCTACATCGATACGATTTAGTAACCGCACATGCCTTCAAGGAGTG20.12376237623762376No Hit
GAGCATCTCTTCAAATATCTGAATTGCTTTTTTTCCATACCCGTGAACGC20.12376237623762376No Hit
AGCAGTGGTAATTCTAAAGATTCCCCCGGGGAAAAATAGAGATGTCTCCT20.12376237623762376No Hit
CTATCATTACAGATTCCATTAAAATACAAACAGAAGCACACAACTAAGTC20.12376237623762376No Hit
AATATGACTTGATCTCATGTGTATGATTGAGTATAAGAACTGAAACCGCA20.12376237623762376No Hit
TATTTTTACACTATTTCGGAAACCCAAGGACTCAATCGTATGGATATGTA20.12376237623762376No Hit
GTTATTTTTAAAAAAATATATAGCCCCGCGATATATGACGGGTTAAAATC20.12376237623762376No Hit
ATTGATTATTGGAATAGATTTTTTAAGCTATTATGAATTATCCCCAAAAA20.12376237623762376No Hit
TTTTCTTTTAATATGTTGATTATTCCAAAAGAGATTTTCGTTATTTTAGT20.12376237623762376No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)