FastQCFastQC Report
Wed 27 Mar 2019
H5NLTBGXB_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5NLTBGXB_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40671358
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17084754.200683439190794No Hit
GTATATTTGGCTCGAAAACTGCCTTATTAACAAATTTTGGCAAAATGAAA1469570.3613279890973889No Hit
TTTAGGGTCTCCGCATAACACCCAATTTCCATTTCCAAATTACCCTCACC1275000.31348842593355253No Hit
TGGCGTTAAATTCTAGGTGGAAGTCGTGCATTTATTGAAGGCCGTTTATG1136060.2793267930714288No Hit
GAAAGTGGTAAGGTTGGAGAAGAATAAGAATAAAATAACGGAAAGATGTC1130730.2780162885143889No Hit
ATGTGTTTTGACCCGAGTAATCCACAACAACAACGACCACCTGTCAATCC863730.21236812402477437No Hit
GGTTCAAATCAGGTCCATTTTGCCACTCAATCATAAAAATGGACGTTTCA834760.20524517524101357No Hit
CCATTTTTATGATTGAGTGGCAAAATGGACCTGATTTGAACCGAGATAGA777820.191245150948734No Hit
CATGTAAAGAGTTTTTTAGGATCAATATCACTTTAAAATAAGGTACAGTC591800.1455078042882168No Hit
CATTTTGACTGAACTGGAGAACAGTGTTCGAAATATATTTCAACTGTACT538140.13231424433873096No Hit
TCATGCATCCAAATACAATGATCCAGGGAAAAAGACATTGAAGGGATGAA461160.11338691961060164No Hit
GGTTCAAATCAGGTCCATTTTGCAATTGAATTTCGTAGCAAAAATCCAAA420930.10349543774761591No Hit
GAATTTTATTAGACCTTGAGATCTTCTTATCTGTGCAACTGATGATATTT420050.10327906926540294No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTGGG185600.067.1032114
AACGAGG193900.067.029161
ACGAGGT199650.066.405742
CCTGGGA187800.066.2613215
TAGGATC154550.066.1210517
CTGGGAT191650.064.8939416
CTAGGTG143100.064.7348913
TGGCGTT133900.064.7097551
TGGGATC191950.064.6837217
CATGTAA181750.064.658221
GTCGTGC139900.064.4916223
CCTTGAG143000.064.3414714
GTGTTCG171550.064.2575524
GTAAAGA190100.064.2081454
TGTAAAG188000.064.0884553
CGACTTA135850.063.89509264
TTAGGAT190700.063.6762316
GGTCGAC135100.063.6462661
ACTGTAC172950.063.6433843
TGACCCG165300.063.638299
Produced by FastQC (version 0.11.5)