FastQCFastQC Report
Wed 27 Mar 2019
H5NLTBGXB_n01_JK05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5NLTBGXB_n01_JK05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24984654
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTTTGACTGAACTGGAGAACAGTGTTCGAAATATATTTCAACTGTACT388284015.540899625826318No Hit
CATTTTGACTGAACTGGAGAACAGTGTTCGAAATATATTTCAACTGTACA279610511.19128966124566No Hit
CATTTTGACTGAACTGGAGAACAGTGTTCGAAATATATTTCAACTGTACC24466639.792663128334697No Hit
CATTTTGACTGAACTGGAGAACAGTGTTCGAAATATATTTCAACTGTACG9205133.6843135790473625No Hit
AACGAGGTAAACTAATTTTGATCGCAATCGACGTCGTCAAGCTGGCAAAA2855721.1429896127438868No Hit
AACGAGGTAAACTAATTTTGATCTAGTTTGGTTAAGCGCTTAATTTGAAG2852071.1415287159870213No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG410620.16434888391890476No Hit
CATTTTGACTGAACTGGAGAACAGATCAAAATTAGTTTACCTCGTTAGAT361400.14464879121399882No Hit
AACGAGGTAAACTAATTTTGATCTGTTCTCCAGTTCAGTCAAAATGAGAT337670.1351509610659407No Hit
AACGAGGTAAACTAATTTTGATCAGGACAGTTTGAGATATTGAAGAAAAT289150.11573104034180341No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGAGG12859650.069.815281
ACGAGGT13065700.068.820372
CGAGGTA13172400.068.559443
CATTTTG11073950.068.402421
GAGGTAA13227750.068.268594
GGTAAAC13250550.068.194446
AGGTAAA13257150.068.121165
GTAAACT13284700.068.0225147
TTTGATC12917350.067.9957117
TAAACTA13301050.067.9760068
AACTAAT13324950.067.8585410
ACTAATT13359200.067.7044811
TTGATCT3945750.067.66942618
AAACTAA13388300.067.589229
CTAATTT13396150.067.5449112
TTGATCC2250050.067.4645818
TGTACGC100050.067.4455545
CTGTACT4216400.067.396144
TTGATCA4500800.067.35998518
TTGATCG2327450.067.3580218