FastQCFastQC Report
Wed 27 Mar 2019
H5NLTBGXB_n01_DJ29.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5NLTBGXB_n01_DJ29.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16225759
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTAGGGTCTCCGCATAACACCCAATTTCCATTTCCAAATTACCCTCACC679001041.84710249918047No Hit
GAATTTTATTAGACCTTGAGATCTTCTTATCTGTGCAACTGATGATATTT225685813.909105885277848No Hit
TTAGGGTCTCCGCATAACACCCAATTTCCATTTCCAAATTACCCTCACCT5410133.334284701258043No Hit
TTTAGGGTCTCCGCATAACACCCAATTTCCATTTCCAAATTACCCTCCCC1942951.1974478358762755No Hit
GGGTCTCCGCATAACACCCAATTTCCATTTCCAAATTACCCTCACCTACC1794941.1062286824302026No Hit
TTTAGGGTCTCCGCATAACACCCTAAAGATCTCAAGGTCTAATAAAATTC1209880.7456538704907425No Hit
AGGGTCTCCGCATAACACCCAATTTCCATTTCCAAATTACCCTCACCTAC984770.6069176794749632No Hit
GGTCTCCGCATAACACCCAATTTCCATTTCCAAATTACCCTCACCTACCT785280.4839711966632809No Hit
GAATTTTATTAGACCTTGAGATCTTTAGGGTGTTATGCGGAGACCCTAAA756850.46644967424944495No Hit
TTTAGGGTCTCCGCATACCACCCAATTTCCATTTCCAAATTACCCTCACC603140.3717175880647555No Hit
GTTAGGGTCTCCGCATAACACCCAATTTCCATTTCCAAATTACCCTCACC568700.3504920786756416No Hit
TTTAGGGTCTCCGCATAACCCCCAATTTCCATTTCCAAATTACCCTCACC445030.27427376432744993No Hit
GAATTTTATTAGCCCTTGAGATCTTCTTATCTGTGCAACTGATGATATTT412790.2544041237146441No Hit
TAGGGTCTCCGCATAACACCCAATTTCCATTTCCAAATTACCCTCACCTA378850.23348676632014564No Hit
GTCTCCGCATAACACCCAATTTCCATTTCCAAATTACCCTCACCTACCTG260370.1604670696760626No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG259240.15977064616823164No Hit
TTAGGGTCTCCGCATAACACCCAATTTCCATTTCCAAATTACCCTCCCCT250260.1542362363449377No Hit
TTTAGGGTCTCCGCATAACACCCAGATCTCAAGGTCTAATAAAATTCAGA242050.1491763805933516No Hit
GGATTTTATTAGACCTTGAGATCTTCTTATCTGTGCAACTGATGATATTT183200.11290689082711015No Hit
GAATTTTATTAGACCTTGAGATCGCCGTGAATGGAACGGCATGTATTGGA171790.10587486230998501No Hit
AATTTTATTAGACCTTGAGATCTTCTTATCTGTGCAACTGATGATATTTG165780.10217087533470699No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTACGT150.002225542769.9991268
GAATTTT6714250.069.097541
TTTAGGG7791100.068.8613361
TTAGACC6736000.068.853949
TAGACCT6739700.068.83349610
GACCTTG6737900.068.82767512
AGACCTT6739800.068.8207411
ATTAGAC6749250.068.820418
TGAGATC6823450.068.77904517
CCTTGAG6847800.068.778814
TCCGGTA2458800.068.7422270
GAGATCT3623100.068.7269518
AATTTTA6759900.068.71892
TCTGTGC2499750.068.7054130
CTTATCT2498700.068.6937426
CTTGAGA6830600.068.68143515
GTTCCGG2486850.068.6733668
TGCTCAG2496300.068.66576455
TATCTGT2501000.068.6628828
GTGCAAC2482800.068.65710433
Produced by FastQC (version 0.11.5)