FastQCFastQC Report
Wed 27 Mar 2019
H5NLTBGXB_n01_DJ28.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5NLTBGXB_n01_DJ28.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16129663
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTGTTTTGACCCGAGTAATCCACAACAACAACGACCACCTGTCAATCC544833133.77833126457757No Hit
CATGTAAAGAGTTTTTTAGGATCAATATCACTTTAAAATAAGGTACAGTC532701233.02618287809237No Hit
ATGTGTTTTGACCCGAGTAATCCTAATCCTAAAAAACTCTTTACATGTCC2258111.399973452638161No Hit
CATGTAAAGAGTTTTTTAGGATTAGGATTACTCGGGTCAAAACACATTTG2048511.270026534342348No Hit
ATGTGTTTTGACCCGAGTAATCCTAATCCTAAAAAACTCTTTACATGAGA2047371.2693197619813879No Hit
ATGTGTTTTGACCCGAGTAATCCACAACAACAACGCCCACCTGTCAATCC1919971.190334850765326No Hit
CATGTAAAGAGTTTTTTAGGATTAGGATTACTCGGGTCAAAACACATAGA1557810.9658044312519114No Hit
GTGTGTTTTGACCCGAGTAATCCACAACAACAACGACCACCTGTCAATCC1345140.833954187387548No Hit
ATGTGTTTTGCCCCGAGTAATCCACAACAACAACGACCACCTGTCAATCC704350.43667992319492355No Hit
TGTTTTGACCCGAGTAATCCACAACAACAACGACCACCTGTCAATCCGTA631380.39144029233592786No Hit
GTGTTTTGACCCGAGTAATCCACAACAACAACGACCACCTGTCAATCCGT613950.38063411492230187No Hit
GTTTTGACCCGAGTAATCCACAACAACAACGACCACCTGTCAATCCGTAG609050.37759623372168405No Hit
TGTGTTTTGACCCGAGTAATCCACAACAACAACGACCACCTGTCAATCCG603270.3740127738564656No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT564040.34969112497886656TruSeq Adapter, Index 20 (97% over 44bp)
ATGTGTTTTGACCCGAGTAATCCACAACAACAACGACCCCCTGTCAATCC490240.3039369142430316No Hit
CATGTAAAGAGTTTTTTAGGATTACTCGGGTCAAAACACATTTGCAAAAA451030.2796276648805372No Hit
ATGTGTTTTGACCCGAGTAATCCACAACAACACCGACCACCTGTCAATCC450430.2792556794274003No Hit
ATGTGTTTTGACCCGAGTAATCCGATCCTAAAAAACTCTTTACATGAGAT373270.2314183501539989No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG372560.2309781673677869No Hit
CATGTAAAGAGTTTTTTAGGATCGGATTACTCGGGTCAAAACACATAGAT345870.21443101446074847No Hit
ATGTGTTTTGACCCGAGTAATCCTAGATCCTAAAAAACTCTTTACATGAG283750.1759181205459779No Hit
CATGTAAAGAGTTTTTTAGGATCACTGGTTTGTCGCTAGAAACTTTCTGT253750.15731884788913444No Hit
ATGTGTTTTGACCCGAGTAATCCTAAAAAACTCTTTACATGTCCGCAAGG253630.15724445079850707No Hit
TTTTGACCCGAGTAATCCACAACAACAACGACCACCTGTCAATCCGTAGG228410.14160866225165397No Hit
ATGTGTTTTGACCCGAGTAATCCCCAACAACAACGACCACCTGTCAATCC227740.14119327849565116No Hit
CATGTAAAGAGTTTTTTAGGATCTAGGATTACTCGGGTCAAAACACATAG205290.12727482279077995No Hit
GTGTGTTTTGACCCGAGTAATCCACAACAACAACGCCCACCTGTCAATCC187760.11640664780163107No Hit
ATGTAAAGAGTTTTTTAGGATCAATATCACTTTAAAATAAGGTACAGTCT176400.10936372322223967No Hit
CATGTAAAGAGTTTTTTAGGATCAATATCCCTTTAAAATAAGGTACAGTC166360.10313916663974938No Hit
ATGTGTTTGACCCGAGTAATCCACAACAACAACGACCACCTGTCAATCCG166160.10301517148870376No Hit
CATGTAAAGAGTTTTTTAGGATTACTCGGGTCAAAACACATAGATCGGAA164660.10208520785586159No Hit
ATGTGTTTTGACCCGAGTAATCCACAACACCAACGACCACCTGTCAATCC163040.10108084713239204No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGTAA7732100.069.833371
ATGTGTT6918750.069.80141
GGTACCA19650.069.6431342
ATGTAAA7763150.069.605672
TGTAAAG7805450.069.357153
GATCAAT5929150.069.3172420
GTAAAGA7863400.069.312054
GGATCAA6083700.069.31106619
TAAGGTA5855500.069.30360439
TAAAGAG7869050.069.295655
ATAAGGT5872300.069.2948738
CACTTTA5836350.069.2827329
TATCACT5831750.069.2811826
ATATCAC5836350.069.2639725
AATAAGG5884450.069.26063537
CAATATC5865500.069.25915523
ATCAATA5897500.069.2497421
ACTTTAA5853750.069.2489930
TAGGATC7410400.069.2367917
TCAATAT5889750.069.2271522
Produced by FastQC (version 0.11.5)